Summary of Study ST000164
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000142. The data can be accessed directly via it's Project DOI: 10.21228/M8MG6K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000164 |
| Study Title | Metabolomic analysis of normal and diabetic mouse bone marrow under PBS or metformin treatment |
| Study Type | Untargeted Metabolomics |
| Study Summary | Wild type and diabetic (MKR) mice were treated daily with intraperitoneal injections of 50 microliters of PBS (control) or metformin (200 mg/kg body weight) for 14 days. Their bone marrow cells were collected and compared by untargeted metabolomics. |
| Institute | New York University |
| Department | College of Dentistry, Basic Science and Craniofacial Biology |
| Laboratory | Xin Li Laboratory |
| Last Name | Xin |
| First Name | Li |
| Address | 345 East 24th Street, Room 901D, New York, NY 10010 |
| xl15@nyu.edu | |
| Phone | 1-(212)992-7009 |
| Submit Date | 2015-03-04 |
| Num Groups | 4 |
| Total Subjects | 15 samples |
| Study Comments | each sample with 3 technical replicates in each mode |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 7.1 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000256 | AN000257 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Exactive | Thermo Exactive |
| Column | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000181 |
| Chromatography Summary: | Untarged mix-mode method |
| Instrument Name: | Thermo Exactive |
| Column Name: | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
| Column Pressure: | 150 Bar (Max) |
| Column Temperature: | 45 |
| Flow Gradient: | 0-15 min -1% B, 15-19 min- gradient 1 to 99% B, 18-24 min- 99% B, 24-25 min-gradient 99% to 1% B, 25-30 min-1% B |
| Flow Rate: | 0.1 ml/min |
| Injection Temperature: | 4 C |
| Sample Injection: | 5 uL |
| Solvent A: | 10% acetonitrile/90% water; 10 mM ammonium acetate ; 10 mM acetic acid (positive) or 10 mM NH3.H2O (negative) |
| Solvent B: | 90% acetonitrile/10% water; 10 mM ammonium acetate; 10 mM acetic acid (positive) or 10 mM ammonia (negative) |
| Analytical Time: | 30 min |
| Capillary Voltage: | 3.5 kv (positive), 3.1 kv (negative) |
| Transferline Temperature: | 275C |
| Washing Buffer: | 10% Acetonitrile |
| Weak Wash Solvent Name: | 10% Acetonitrile |
| Weak Wash Volume: | 600 uL |
| Strong Wash Solvent Name: | 90% Acetonitrile |
| Strong Wash Volume: | 200 uL |
| Sample Loop Size: | 20 uL |
| Chromatography Type: | Reversed phase |
