Summary of study ST000164

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000142. The data can be accessed directly via it's Project DOI: 10.21228/M8MG6K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data (Contains raw data)
Study IDST000164
Study TitleMetabolomic analysis of normal and diabetic mouse bone marrow under PBS or metformin treatment
Study TypeUntargeted Metabolomics
Study SummaryWild type and diabetic (MKR) mice were treated daily with intraperitoneal injections of 50 microliters of PBS (control) or metformin (200 mg/kg body weight) for 14 days. Their bone marrow cells were collected and compared by untargeted metabolomics.
Institute
New York University
DepartmentCollege of Dentistry, Basic Science and Craniofacial Biology
LaboratoryXin Li Laboratory
Last NameXin
First NameLi
Address345 East 24th Street, Room 901D, New York, NY 10010
Emailxl15@nyu.edu
Phone1-(212)992-7009
Submit Date2015-03-04
Num Groups4
Total Subjects15 samples
Study Commentseach sample with 3 technical replicates in each mode
Raw Data AvailableYes
Raw Data File Type(s).raw
Uploaded File Size7.1 G
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Li Xin Li Xin
https://dx.doi.org/10.21228/M8MG6K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Combined analysis:

Analysis ID AN000256 AN000257
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Exactive Thermo Exactive
Column Shodex ODP2 HP-4B 5um polymer 4.6 x 50 mm Shodex ODP2 HP-4B 5um polymer 4.6 x 50 mm
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH000181
Chromatography Summary:Untarged mix-mode method
Instrument Name:Thermo Exactive
Column Name:Shodex ODP2 HP-4B 5um polymer 4.6 x 50 mm
Column Pressure:150 Bar (Max)
Column Temperature:45
Flow Gradient:0-15 min -1% B, 15-19 min- gradient 1 to 99% B, 18-24 min- 99% B, 24-25 min-gradient 99% to 1% B, 25-30 min-1% B
Flow Rate:0.1 ml/min
Injection Temperature:4 C
Sample Injection:5 uL
Solvent A:10% Acetonitrile + 10 mM NH4OAc + 10 mM Acetic acid (positive) or 10 mM NH3.H2O (negative)
Solvent B:90% Acetonitrile + 10 mM NH4OAc + 10 mM Acetic acid (positive) or 10 mM NH3.H2O (negative)
Analytical Time:30 min
Capillary Voltage:3.5 kv (positive), 3.1 kv (negative)
Transferline Temperature:275C
Washing Buffer:10% Acetonitrile
Weak Wash Solvent Name:10% Acetonitrile
Weak Wash Volume:600 uL
Strong Wash Solvent Name:90% Acetonitrile
Strong Wash Volume:200 uL
Sample Loop Size:20 uL
Chromatography Type:Reversed phase
  logo