Summary of Study ST000164
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000142. The data can be accessed directly via it's Project DOI: 10.21228/M8MG6K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000164 |
Study Title | Metabolomic analysis of normal and diabetic mouse bone marrow under PBS or metformin treatment |
Study Type | Untargeted Metabolomics |
Study Summary | Wild type and diabetic (MKR) mice were treated daily with intraperitoneal injections of 50 microliters of PBS (control) or metformin (200 mg/kg body weight) for 14 days. Their bone marrow cells were collected and compared by untargeted metabolomics. |
Institute | New York University |
Department | College of Dentistry, Basic Science and Craniofacial Biology |
Laboratory | Xin Li Laboratory |
Last Name | Xin |
First Name | Li |
Address | 345 East 24th Street, Room 901D, New York, NY 10010 |
xl15@nyu.edu | |
Phone | 1-(212)992-7009 |
Submit Date | 2015-03-04 |
Num Groups | 4 |
Total Subjects | 15 samples |
Study Comments | each sample with 3 technical replicates in each mode |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Uploaded File Size | 7.1 G |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000256 | AN000257 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Exactive | Thermo Exactive |
Column | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH000181 |
Chromatography Summary: | Untarged mix-mode method |
Instrument Name: | Thermo Exactive |
Column Name: | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
Column Pressure: | 150 Bar (Max) |
Column Temperature: | 45 |
Flow Gradient: | 0-15 min -1% B, 15-19 min- gradient 1 to 99% B, 18-24 min- 99% B, 24-25 min-gradient 99% to 1% B, 25-30 min-1% B |
Flow Rate: | 0.1 ml/min |
Injection Temperature: | 4 C |
Sample Injection: | 5 uL |
Solvent A: | 10% acetonitrile/90% water; 10 mM ammonium acetate ; 10 mM acetic acid (positive) or 10 mM NH3.H2O (negative) |
Solvent B: | 90% acetonitrile/10% water; 10 mM ammonium acetate; 10 mM acetic acid (positive) or 10 mM ammonia (negative) |
Analytical Time: | 30 min |
Capillary Voltage: | 3.5 kv (positive), 3.1 kv (negative) |
Transferline Temperature: | 275C |
Washing Buffer: | 10% Acetonitrile |
Weak Wash Solvent Name: | 10% Acetonitrile |
Weak Wash Volume: | 600 uL |
Strong Wash Solvent Name: | 90% Acetonitrile |
Strong Wash Volume: | 200 uL |
Sample Loop Size: | 20 uL |
Chromatography Type: | Reversed phase |