Summary of study ST000164

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000142. The data can be accessed directly via it's Project DOI: 10.21228/M8MG6K This work is supported by NIH grant, U2C- DK119886.

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Study IDST000164
Study TitleMetabolomic analysis of normal and diabetic mouse bone marrow under PBS or metformin treatment
Study TypeUntargeted Metabolomics
Study SummaryWild type and diabetic (MKR) mice were treated daily with intraperitoneal injections of 50 microliters of PBS (control) or metformin (200 mg/kg body weight) for 14 days. Their bone marrow cells were collected and compared by untargeted metabolomics.
Institute
New York University
DepartmentCollege of Dentistry, Basic Science and Craniofacial Biology
LaboratoryXin Li Laboratory
Last NameXin
First NameLi
Address345 East 24th Street, Room 901D, New York, NY 10010
Emailxl15@nyu.edu
Phone1-(212)992-7009
Submit Date2015-03-04
Num Groups4
Total Subjects15 samples
Study Commentseach sample with 3 technical replicates in each mode
Raw Data AvailableYes
Raw Data File Type(s).raw
Uploaded File Size7.1 G
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Li Xin Li Xin
https://dx.doi.org/10.21228/M8MG6K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000256 AN000257
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Exactive Thermo Exactive
Column Shodex ODP2 HP-4B 5um polymer 4.6 x 50 mm Shodex ODP2 HP-4B 5um polymer 4.6 x 50 mm
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

MS:

MS ID:MS000206
Analysis ID:AN000256
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:30V
Collision Gas:40 units
Ion Source Temperature:3.5 kv
Ion Spray Voltage:Electrospray Ionization
Ionization Potential:< 2ppm
Atom Gun Current:3000000
Sample Dripping:75-1200
Scanning Range:20 v
Skimmer Voltage:120 v
  
MS ID:MS000207
Analysis ID:AN000257
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Temperature:30V
Collision Gas:40 units
Ion Source Temperature:3.1 kv
Ion Spray Voltage:Electrospray Ionization
Ionization Potential:< 4 ppm
Atom Gun Current:3000000
Sample Dripping:75-1200
Scanning Range:20 v
Skimmer Voltage:120 v
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