Summary of study ST000164

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000142. The data can be accessed directly via it's Project DOI: 10.21228/M8MG6K This work is supported by NIH grant, U2C- DK119886.

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Study IDST000164
Study TitleMetabolomic analysis of normal and diabetic mouse bone marrow under PBS or metformin treatment
Study TypeUntargeted Metabolomics
Study SummaryWild type and diabetic (MKR) mice were treated daily with intraperitoneal injections of 50 microliters of PBS (control) or metformin (200 mg/kg body weight) for 14 days. Their bone marrow cells were collected and compared by untargeted metabolomics.
Institute
New York University
DepartmentCollege of Dentistry, Basic Science and Craniofacial Biology
LaboratoryXin Li Laboratory
Last NameXin
First NameLi
Address345 East 24th Street, Room 901D, New York, NY 10010
Emailxl15@nyu.edu
Phone1-(212)992-7009
Submit Date2015-03-04
Num Groups4
Total Subjects15 samples
Study Commentseach sample with 3 technical replicates in each mode
Raw Data AvailableYes
Raw Data File Type(s).raw
Uploaded File Size7.1 G
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Li Xin Li Xin
https://dx.doi.org/10.21228/M8MG6K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000183
Sampleprep Summary:Frozen cells were mixed with dry ice-cool 80% methanol (mass-spec grade) at a ratio of 30 ?L/million cells, and then quickly thawed on heat block set at 50 °C for 5 min. The suspension was then processed by three rounds of 1 min vortex at max speed, chilled briefly on dry ice. The mixture was incubated at 4°C for 1 hour before centrifuge at 20,000 x g for 20 min at 4 °C. The supernatant was stored at -20 °C and used as metabolite extract for LC-MS analysis.
Sampleprep Protocol Filename:Protocol_Methods_metformin_Li.pdf
Processing Method:Homogenization and Centrifugation
Processing Storage Conditions:On ice
Extraction Method:80% Methanol
Extract Concentration Dilution:30 microliters solvent per million cells
Extract Storage:-20°C
Cell Type:bone marrow
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