Summary of Study ST000164
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000142. The data can be accessed directly via it's Project DOI: 10.21228/M8MG6K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000164 |
| Study Title | Metabolomic analysis of normal and diabetic mouse bone marrow under PBS or metformin treatment |
| Study Type | Untargeted Metabolomics |
| Study Summary | Wild type and diabetic (MKR) mice were treated daily with intraperitoneal injections of 50 microliters of PBS (control) or metformin (200 mg/kg body weight) for 14 days. Their bone marrow cells were collected and compared by untargeted metabolomics. |
| Institute | New York University |
| Department | College of Dentistry, Basic Science and Craniofacial Biology |
| Laboratory | Xin Li Laboratory |
| Last Name | Xin |
| First Name | Li |
| Address | 345 East 24th Street, Room 901D, New York, NY 10010 |
| xl15@nyu.edu | |
| Phone | 1-(212)992-7009 |
| Submit Date | 2015-03-04 |
| Num Groups | 4 |
| Total Subjects | 15 samples |
| Study Comments | each sample with 3 technical replicates in each mode |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 7.1 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000183 |
| Sampleprep Summary: | Frozen cells were mixed with dry ice-cool 80% methanol (mass-spec grade) at a ratio of 30 ?L/million cells, and then quickly thawed on heat block set at 50 °C for 5 min. The suspension was then processed by three rounds of 1 min vortex at max speed, chilled briefly on dry ice. The mixture was incubated at 4°C for 1 hour before centrifuge at 20,000 x g for 20 min at 4 °C. The supernatant was stored at -20 °C and used as metabolite extract for LC-MS analysis. |
| Sampleprep Protocol Filename: | Protocol_Methods_metformin_Li.pdf |
| Processing Method: | Homogenization and Centrifugation |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 80% Methanol |
| Extract Concentration Dilution: | 30 microliters solvent per million cells |
| Extract Storage: | -20°C |
| Cell Type: | bone marrow |
