Summary of Study ST000465

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000357. The data can be accessed directly via it's Project DOI: 10.21228/M8WW32 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000465
Study TitleUniquely Tumor-Selective Englerin A Profoundly Alters Lipid Metabolism in Renal Cell Carcinoma inducing ER-Stress and an Acute Inflammatory Response
Study TypeMetabolomic effect of Englerin A on renal cell carcinoma
Study SummaryThis targeted metabolomic analysis was performed on renal cell carcinoma A498 cells with or without anti-cancer drug Englerin treatment for 24 and 48 h.
Institute
University of California, San Diego
DepartmentDepartment of Pediatrics
Last NameBatova
First NameAyse
AddressLa Jolla, CA 92093
Emailabatova@ucsd.edu
Phone619-543-1962
Submit Date2016-09-09
Num GroupsTwo groups for 24 h treatment (control and Eglerin treatment) and two groups for 48 h treatment. Each has 4 replicates
Total Subjects16
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Ayse Batova Ayse Batova
https://dx.doi.org/10.21228/M8WW32
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000726 AN000727
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Shimadzu 20AD Shimadzu 20AD
Column Luna NH2 aminopropyl HPLC column Luna NH2 aminopropyl HPLC column
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap
Ion Mode NEGATIVE POSITIVE
Units AUC AUC

Chromatography:

Chromatography ID:CH000520
Chromatography Summary:HILIC
Instrument Name:Shimadzu 20AD
Column Name:Luna NH2 aminopropyl HPLC column
Column Temperature:25C
Flow Gradient:0 min—95% B, 4 min—B, 19 min—2% B, 22 min—2% B, 23 min—95% B, 28 min—end.
Flow Rate:0.3 ml/min
Solvent A:95% water with 23.18mM NH4OH+20 mM formic acid (Ph 9.4)
Solvent B:100% acetonitirle
Analytical Time:28 min
Oven Temperature:25C
Target Sample Temperature:4C
Sample Loop Size:10 ul
Sample Syringe Size:100 ul
Chromatography Type:HILIC
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