Summary of Study ST000617

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000451. The data can be accessed directly via it's Project DOI: 10.21228/M8RP6W This work is supported by NIH grant, U2C- DK119886.


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Study IDST000617
Study TitleValidation of the application of targeted metabolomic appraoch in the diagnosis of CFS
Study TypePlasma metabolomic profiling
Study SummaryThis study was to validate the utility of the developed targeted metabolomic method in the diagnosis of chronic fatigue syndrome (CFS). Clinical validation consisted of a cohort of 20 male CFS (53 ± 2.8 years old, mean ± SEM, range 21-67 y) and 18 male controls (53 ± 3.5 years old, mean ± SEM, range 23-69 y), who were enrolled in a previous study (Naviaux et al. 2016). These plasma samples were stored in -80 ºC for about 1.5 years and reanalyzed on a different LC-MS/MS system by a different investigator.
University of California, San Diego
DepartmentDepartment of Medicine
LaboratoryThe Mitochondrial and Metabolic Disease Center
Last NameNaviaux
First NameRobert
Address214 Dikinson Street, CTF-C102, San Diego, CA, 92103
Submit Date2017-05-17
Num Groups2
Total Subjects38
Num Males38
Study CommentsThese plasma samples were stored in -80 ºC for about 1.5 years and reanalyzed on a different LC-MS/MS system by a different investigator.
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-06-17
Release Version1
Robert Naviaux Robert Naviaux application/zip

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Combined analysis:

Analysis ID AN000946 AN000947
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Shimadzu 20AD Shimadzu 20AD
Column Shodex NH2P-40 2E (250× 2.0 mm) Shodex NH2P-40 2E (250× 2.0 mm)
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap


Chromatography ID:CH000674
Chromatography Summary:hydrophilic interaction chromatography (HILIC) on shodex column
Instrument Name:Shimadzu 20AD
Column Name:Shodex NH2P-40 2E (250× 2.0 mm)
Column Pressure:900~2800 psi
Column Temperature:25 C
Flow Gradient:0-3.5 min: 95%B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 13.5–35 min: 0% B, 36–46 min: 95% B, 46.1 min: end.
Flow Rate:0.2 ml/min
Injection Temperature:room temperature
Solvent A:95% water/5% acetonitrile; 20 mM ammonium carbonate; 38 mM ammonium hydroxide, pH 9.8
Solvent B:100% acetonitrile
Analytical Time:46.1 min
Oven Temperature:25 C
Sample Loop Size:10 ul
Sample Syringe Size:100 ul
Chromatography Type:HILIC