Summary of Study ST000617

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000451. The data can be accessed directly via it's Project DOI: 10.21228/M8RP6W This work is supported by NIH grant, U2C- DK119886.


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Study IDST000617
Study TitleValidation of the application of targeted metabolomic appraoch in the diagnosis of CFS
Study TypePlasma metabolomic profiling
Study SummaryThis study was to validate the utility of the developed targeted metabolomic method in the diagnosis of chronic fatigue syndrome (CFS). Clinical validation consisted of a cohort of 20 male CFS (53 ± 2.8 years old, mean ± SEM, range 21-67 y) and 18 male controls (53 ± 3.5 years old, mean ± SEM, range 23-69 y), who were enrolled in a previous study (Naviaux et al. 2016). These plasma samples were stored in -80 ºC for about 1.5 years and reanalyzed on a different LC-MS/MS system by a different investigator.
University of California, San Diego
DepartmentDepartment of Medicine
LaboratoryThe Mitochondrial and Metabolic Disease Center
Last NameNaviaux
First NameRobert
Address214 Dikinson Street, CTF-C102, San Diego, CA, 92103
Submit Date2017-05-17
Num Groups2
Total Subjects38
Num Males38
Study CommentsThese plasma samples were stored in -80 ºC for about 1.5 years and reanalyzed on a different LC-MS/MS system by a different investigator.
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-06-17
Release Version1
Robert Naviaux Robert Naviaux application/zip

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Sample Preparation:

Sampleprep ID:SP000647
Sampleprep Summary:Metabolites from plasma were extracted using pre-chilled methanol/acetonitrile (50:50)
Extraction Method:Typically, 90 μl of plasma was thawed on ice and mixed with 5 μl of isotope standards and incubated for 10 min at 20°C to permit small molecules and vitamins in the internal standards to associate with plasma binding proteins. Macromolecules (protein, DNA, RNA, glycans, etc.) were precipitated by extraction with 4 volumes (400 μl) of cold (-20°C), acetonitrile:methanol (50:50) and incubated on crushed ice for 10 min, then removed by centrifugation at 16,000g x 10 min at 4°C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80°C for LCMS/MS analysis.
Extract Storage:-80
Sample Derivatization:No
Sample Spiking:25-35 commercial stable isotope internal standards, and 57 stable isotope internal standards that were custom-synthesized in E. coli and S. cerevisiae by metabolic labeling with 13C-glucose and 13C bicarbonate