Summary of Study ST000883
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000612. The data can be accessed directly via it's Project DOI: 10.21228/M8FM7S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000883 |
| Study Title | Breathprinting Reveals Malaria-Associated Biomarkers and Mosquito Attractants |
| Study Summary | Current evidence suggests that malaria infection could alter patient breath metabolites, a phenomenon that could be exploited to create a breath-based diagnostic test. Indications include the preferential attraction of the Anopheles mosquito vector upon infection and a distinct breath profile with the progression of experimental, sub-microscopic malaria. However, these observations have yet to be extended to the clinic. To investigate whether natural human malaria infection leads to a characteristic breath profile, we performed a field study in Malawi. Breath volatiles from pediatric patients with and without uncomplicated falciparum malaria were analyzed by thermal desorption-gas chromatography/mass spectrometry. Using an unbiased, correlation-based analysis, we find that children with malaria have a distinct shift in overall breath composition. Leveraging these differences, highly accurate classification of infection status was achieved with a suite of six compounds. In addition, we find that malaria-infected children have significantly higher breath levels of two mosquito-attractant terpenes, α-pinene and 3-carene. Thus, our work attests to the viability of breath analysis for malaria diagnosis, identifies candidate compounds for follow-up studies, and identifies biologically plausible chemical mediators for increased mosquito attraction to malaria-infected patients. |
| Institute | Washington University in St. Louis |
| Department | School of Medicine |
| Last Name | Schaber |
| First Name | Chad |
| Address | 4938 Parkview Place, MPRB/FLoor 6, Entry 5, St. Louis, MO, 63110, USA |
| chadschaber@wustl.edu | |
| Phone | 3142862040 |
| Submit Date | 2017-10-08 |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-02-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001440 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus 4D GC |
| Column | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF |
| Ion Mode | POSITIVE |
| Units | raw abundance |
Chromatography:
| Chromatography ID: | CH001011 |
| Chromatography Summary: | All samples were run with a TurboMatrix 650 ATD (Perkin Elmer) connected to a Leco Pegasus 4D GCxGC-TOFMS system. Before analysis, sorbent tubes were brought to room temperature and purged for 5 min with BiP N2 (Airgas) at 60 mL/min. A gaseous standard mixture (20.1 ng fluorobenzene, 18.6 ng toluene-D8, 21.7 ng bromofluorobenzene, 20.3 ng 1,2-dichlorobenzene-D4) was added to each tube by the TurboMatrix 650 immediately prior to analysis. Tubes were desorbed at 270°C, 40 mL/min He flow, with recollection on a secondary Tenax cold trap at 10°C. Analytes were released from the secondary trap by heating to 295°C with 20% transferred to the GC/MS. The GC had a 30 m length x 0.25 mm ID x 0.25 µm film thickness DB-5 column (Agilent). The GC oven was programmed to hold at 40°C for 3 min, ramp 5°C/min to 200°C, then ramp 10°C/min to 250°C, final ramp 25°C/min to 300°C, then hold at 300°C for 3 min. The TOFMS had a sampling frequency of 50 Hz and a mass recording range of 34-400 amu. |
| Instrument Name: | Leco Pegasus 4D GC |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Flow Rate: | 1.2 mL/min |
| Chromatography Type: | GC |
