Summary of Study ST000883
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000612. The data can be accessed directly via it's Project DOI: 10.21228/M8FM7S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000883 |
Study Title | Breathprinting Reveals Malaria-Associated Biomarkers and Mosquito Attractants |
Study Summary | Current evidence suggests that malaria infection could alter patient breath metabolites, a phenomenon that could be exploited to create a breath-based diagnostic test. Indications include the preferential attraction of the Anopheles mosquito vector upon infection and a distinct breath profile with the progression of experimental, sub-microscopic malaria. However, these observations have yet to be extended to the clinic. To investigate whether natural human malaria infection leads to a characteristic breath profile, we performed a field study in Malawi. Breath volatiles from pediatric patients with and without uncomplicated falciparum malaria were analyzed by thermal desorption-gas chromatography/mass spectrometry. Using an unbiased, correlation-based analysis, we find that children with malaria have a distinct shift in overall breath composition. Leveraging these differences, highly accurate classification of infection status was achieved with a suite of six compounds. In addition, we find that malaria-infected children have significantly higher breath levels of two mosquito-attractant terpenes, α-pinene and 3-carene. Thus, our work attests to the viability of breath analysis for malaria diagnosis, identifies candidate compounds for follow-up studies, and identifies biologically plausible chemical mediators for increased mosquito attraction to malaria-infected patients. |
Institute | Washington University in St. Louis |
Department | School of Medicine |
Last Name | Schaber |
First Name | Chad |
Address | 4938 Parkview Place, MPRB/FLoor 6, Entry 5, St. Louis, MO, 63110, USA |
chadschaber@wustl.edu | |
Phone | 3142862040 |
Submit Date | 2017-10-08 |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2018-02-05 |
Release Version | 1 |
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Project:
Project ID: | PR000612 |
Project DOI: | doi: 10.21228/M8FM7S |
Project Title: | Breathprinting Reveals Malaria-Associated Biomarkers and Mosquito Attractants |
Project Summary: | Current evidence suggests that malaria infection could alter patient breath metabolites, a phenomenon that could be exploited to create a breath-based diagnostic test. Indications include the preferential attraction of the Anopheles mosquito vector upon infection and a distinct breath profile with the progression of experimental, sub-microscopic malaria. However, these observations have yet to be extended to the clinic. To investigate whether natural human malaria infection leads to a characteristic breath profile, we performed a field study in Malawi. Breath volatiles from pediatric patients with and without uncomplicated falciparum malaria were analyzed by thermal desorption-gas chromatography/mass spectrometry. Using an unbiased, correlation-based analysis, we find that children with malaria have a distinct shift in overall breath composition. Leveraging these differences, highly accurate classification of infection status was achieved with a suite of six compounds. In addition, we find that malaria-infected children have significantly higher breath levels of two mosquito-attractant terpenes, α-pinene and 3-carene. Thus, our work attests to the viability of breath analysis for malaria diagnosis, identifies candidate compounds for follow-up studies, and identifies biologically plausible chemical mediators for increased mosquito attraction to malaria-infected patients. |
Institute: | Washington University in St. Louis |
Department: | School of Medicine |
Last Name: | Schaber |
First Name: | Chad |
Address: | 4938 Parkview Place, MPRB/FLoor 6, Entry 5, St. Louis, MO, 63110, USA |
Email: | chadschaber@wustl.edu |
Phone: | 3142862040 |