Summary of Study ST001353
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000923. The data can be accessed directly via it's Project DOI: 10.21228/M8CT2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001353 |
Study Title | Untargeted metabolomics in skeletal muscle of mice with chronic kidney disease |
Study Type | MS quantitative analysis |
Study Summary | This study performed untargeted metabolomics analysis of skeletal muscle obtained form mice with and without chronic kidney disease. |
Institute | University of Florida |
Last Name | Ryan |
First Name | Terence |
Address | 1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA |
ryant@ufl.edu | |
Phone | 352-294-1700 |
Submit Date | 2020-04-02 |
Num Groups | 4 |
Total Subjects | 18 |
Num Males | 8 |
Num Females | 10 |
Study Comments | two control male samples processed mistakenly were from soles muscles, while all other samples were gastrocnemius muscles. Due to differences in fiber type proportions, soleus muscles were not used in final analysis |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2020-12-31 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002251 | AN002252 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex | Thermo Dionex |
Column | ACE 5 C18-300 (100 x 2.1mm) | ACE 5 C18-300 (100 x 2.1mm) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak height | peak height |
Chromatography:
Chromatography ID: | CH001655 |
Chromatography Summary: | samples were processed on a Thermo Q-Exactive Oribtrap mass spectrometer with Dionex UHPLC and autosampler. All samples were analyzed in positive and negative heated electrospray ionization with a mass resolution of 35,000 at m/z 200 as separate injections. Separation was achieved on an ACE 18-pfp 100 x 2.1 mm, 2 µm column with mobile phase A as 0.1% formic acid in water and mobile phase B as acetonitrile. This is a polar embedded stationary phase that provides comprehensive coverage, but does have some limitation is the coverage of very polar species. The flow rate was 350 µL/min with a column temperature of 25°C. 4 µL was injected for negative ions and 2 µL for positive ions. |
Instrument Name: | Thermo Dionex |
Column Name: | ACE 5 C18-300 (100 x 2.1mm) |
Column Temperature: | 25C |
Flow Rate: | 350ul/min |
Sample Injection: | 4ul for negative ion, 2ul for positive ion |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |