Summary of Study ST001353

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000923. The data can be accessed directly via it's Project DOI: 10.21228/M8CT2B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001353
Study Title	Untargeted metabolomics in skeletal muscle of mice with chronic kidney disease
Study Type	MS quantitative analysis
Study Summary	This study performed untargeted metabolomics analysis of skeletal muscle obtained form mice with and without chronic kidney disease.
Institute	University of Florida
Last Name	Ryan
First Name	Terence
Address	1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA
Email	ryant@ufl.edu
Phone	352-294-1700
Submit Date	2020-04-02
Num Groups	4
Total Subjects	18
Num Males	8
Num Females	10
Study Comments	two control male samples processed mistakenly were from soles muscles, while all other samples were gastrocnemius muscles. Due to differences in fiber type proportions, soleus muscles were not used in final analysis
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2020-12-31
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001435
Sampleprep Summary:	Muscles were thawed on ice, weighed, and homogenized with a Teflon-tipped conical pestle with a metal rod (Micro-Tube Sample Pestle with Conical Teflon Tip, fits 1.5ml Tubes, autoclavable at 121°F; Research Products International Corp; 199221; Fisher Scientific). The pestle was rinsed with 2-Propanol, water, and methanol and patted dry with a KimWipe in between samples. The samples were centrifuged to pellet the tissue debris and protein concentrations were quantified on the QuBit. The samples were normalized to 500µg/mL of protein with 5mM Ammonium Acetate in water prior to extraction for a total volume of 100µL. 25µL of sample was aliquoted into a clean tube and extracted with 5µL of Global Metabolomics IS and 200µL of 8:1:1 Acetonitrile:Methanol:Acetone to precipitate proteins. The samples were incubated at 4°C for 30 min and centrifuged at 20,000xg at 4°C for 10min. 200µL of supernatant was transferred to a clean Eppendorf tube and dried under nitrogen gas at 30°C and then reconstituted at 25µL in Global Metabolomics Inj. Std. Mix.

Sampleprep ID:

SP001435

Sampleprep Summary:

Muscles were thawed on ice, weighed, and homogenized with a Teflon-tipped conical pestle with a metal rod (Micro-Tube Sample Pestle with Conical Teflon Tip, fits 1.5ml Tubes, autoclavable at 121°F; Research Products International Corp; 199221; Fisher Scientific). The pestle was rinsed with 2-Propanol, water, and methanol and patted dry with a KimWipe in between samples. The samples were centrifuged to pellet the tissue debris and protein concentrations were quantified on the QuBit. The samples were normalized to 500µg/mL of protein with 5mM Ammonium Acetate in water prior to extraction for a total volume of 100µL. 25µL of sample was aliquoted into a clean tube and extracted with 5µL of Global Metabolomics IS and 200µL of 8:1:1 Acetonitrile:Methanol:Acetone to precipitate proteins. The samples were incubated at 4°C for 30 min and centrifuged at 20,000xg at 4°C for 10min. 200µL of supernatant was transferred to a clean Eppendorf tube and dried under nitrogen gas at 30°C and then reconstituted at 25µL in Global Metabolomics Inj. Std. Mix.