Summary of Study ST001795

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001139. The data can be accessed directly via it's Project DOI: 10.21228/M8GH59 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001795
Study TitleChanges in mesenteric lymph lipid profile of mice upon high-fat diet with and without Celecoxib (part I)
Study TypeUntargeted lipidomics analysis
Study SummaryUntargeted lipid profiling of mesenteric mice lymph from mice fed with CHOW, HFD and HFD supplemented with COx-2 inhibitor drug Celecoxib. It is proposed that Celecoxib can protect from deleterious morphological changes in lymphatic system caused by obesity/HFD.
Monash Institute of Pharmaceutical Sciences
DepartmentDrug Delivery, Disposition and Dynamics
LaboratoryTrevaskis Lab, Creek Lab
Last NameAnderson
First NameDovile
Address6 Anderson
Submit Date2021-05-12
Num Groups3
Total Subjects26
Num Males26
PublicationsManuscript NATMETAB-A20022406A Mesenteric lymphatic dysfunction promotes insulin resistance and represents a potential novel treatment target in obesity
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-06-02
Release Version1
Dovile Anderson Dovile Anderson application/zip

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Combined analysis:

Analysis ID AN002915 AN002916
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um) Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um)
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Units height height


Chromatography ID:CH002159
Chromatography Summary:Lipidomics analysis was performed using reversed phase liquid chromatography and high-resolution mass spectrometry. Samples (10 µl) were injected onto a Dionex Ultimate 3000 UHPLC system (Thermo Scientific, Australia) fitted with an analytical C8 column (100 x 2.1 mm; 2.7 µm, Sigma Aldrich, Australia). Chromatography was performed using solvent A (2 mM formic acid, 8 mM ammonium formate, 40% v/v isopropanol) and solvent B (2 mM formic acid, 8 mM ammonium formate, 98% v/v isopropanol) as mobile phases with a 30 min gradient starting at 0% B and increasing to 35% B from 0 to 8 min, then to 50% B from 8-16 min, then to 80% B from 16-19 min, then finally to 100% B by 23 min. 100% B was maintained for a further 3 min before equilibrating to 0% by 28 min and washing for a further 2 min.
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um)
Column Temperature:40C
Flow Gradient:0 min - 0%B, 8 min - 35%B, 16 min - 50%B, 19 min - 80%B, 23 min 100%B, 26 min - 100%B, 28 min - 0%B
Flow Rate:0.2 ml/min
Injection Temperature:4
Solvent A:40% isopropanol/60% water; 2 mM formic acid; 8 mM ammonium formate,
Solvent B:98% isopropanol/2% water; 2 mM formic acid; 8 mM ammonium formate
Analytical Time:30 min
Oven Temperature:40C
Sample Loop Size:25 uL
Sample Syringe Size:25 uL
Chromatography Type:Reversed phase