Summary of Study ST001851
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001168. The data can be accessed directly via it's Project DOI: 10.21228/M8QT2F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001851 |
| Study Title | Quantitative analysis of bile acids in fecal samples from centenarians, elderly and young subjects. |
| Study Summary | Fecal samples from centenarians (>100 yo), elderly (85-89 yo) and young (21-55) subjects were analysed using LC-MS/MS. 48 bile acids were measured by targeted metabolomics. |
| Institute | Keio University School of Medicine |
| Department | Dept of Microbiology and Immunology |
| Last Name | Koji |
| First Name | Atarashi |
| Address | 35 Shinanomachi, Shinjuku-ku, Tokyo, JAPAN |
| kojiatarashi@keio.jp | |
| Phone | 0353633769 |
| Submit Date | 2021-05-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-07-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002999 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Exion LC |
| Column | InertSustain C18 |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex Triple Quad 6500+ |
| Ion Mode | UNSPECIFIED |
| Units | nM |
Chromatography:
| Chromatography ID: | CH002224 |
| Chromatography Summary: | 2 μL was injected to LC/ESI-MS/MS (Triple Quad 6500+ tandem mass spectrometer, equipped with an ESI probe and Exion LC AD ultra-high pressure liquid chromatography system; SCIEX). A separation column, InertSustain C18 (150 mm × 2.1 mm ID, 3 μm particle size; GL Sciences Inc.), was utilized at 40 °C. A mixture of 10 mM ammonium acetate and acetonitrile was used as the eluent and the separation was carried out by linear gradient elution at a flow rate of 0.2 mL/min. The mobile phase composition was gradually changed as follows: ammonium acetate-acetonitrile (86:14, v/v) for 0.5 min, (78:22, v/v) for 0.5-5 min, (72:28, v/v) for 5-28 min, (46:54, v/v) for 28-55 min, (2:98, v/v) for 55-66 min, and (2:98, v/v) for 4 min. The total run time was 70 min. |
| Instrument Name: | Exion LC |
| Column Name: | InertSustain C18 |
| Column Temperature: | 40 |
| Flow Gradient: | ammonium acetate-acetonitrile (86:14, v/v) for 0.5 min, (78:22, v/v) for 0.5-5 min, (72:28, v/v) for 5-28 min, (46:54, v/v) for 28-55 min, (2:98, v/v) for 55-66 min, and (2:98, v/v) for 4 min. The total run time was 70 min. |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 100% water; 10mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
