Summary of Study ST001879

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001185. The data can be accessed directly via it's Project DOI: 10.21228/M8J407 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001879
Study TitleProteomics reveals an increase in the abundance of glycolytic and ethanolic fermentation enzymes in developing sugarcane culms during sucrose accumulation
Study SummarySugarcane is an economically important crop contributing to the world’s sugar and ethanol production with 80% and 40%, respectively. Metabolites from I5-4M and I9-4M were extracted from six biological samples of four-month-old plants. Following the removal of leaves, the internodes were identified and cut, and the bark removed, and the remaining tissue was immediately frozen in liquid nitrogen.
Institute
ESALQ-USP
DepartmentGenetics
LaboratoryLaboratório Max Feffer de Genética de Plantas
Last NameCataldi
First NameThais
AddressPadua Dias Avenue, 11, Piracicaba, São Paulo, 13418-900, Brazil
Emailthais.cataldi@usp.br
Phone+551934294248
Submit Date2021-07-19
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2022-02-16
Release Version1
Thais Cataldi Thais Cataldi
https://dx.doi.org/10.21228/M8J407
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003039
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Agilent DB5 (20m x 0.18 mm i.d. x 0.18 μm)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode UNSPECIFIED
Units relative intensity

Chromatography:

Chromatography ID:CH002254
Chromatography Summary:One microliter of each derivatized sample was injected splitless into a gas chromatograph 7890A (Agilent Technologies, Santa Clara, USA) coupled with a Comb-xt Autosampler (Leap Technologies, Carrboro, USA). A 20m x 0.18 mm i.d. x 0.18 μm film thickness, DB5 GC capillary column (Agilent Technologies) was used as the primary column and the secondary GC columns was a 0.69 m x 0.1 mm i.d. x 0.1 μm film thickness (Rxi-17 Restek, Bellefonte, USA). The injector temperature was 280°C, the septum purge flow rate was 20 ml min-1 and the purge was turned on after 60 s. The gas helium flow rate through the column was 1 ml min-1, the column temperature was held at 80°C for 2 min, then increased by 15°C min-1 to 305°C, and held there for 10 min.
Instrument Name:Agilent 7890A
Column Name:Agilent DB5 (20m x 0.18 mm i.d. x 0.18 μm)
Chromatography Type:GC
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