Summary of Study ST001879

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001185. The data can be accessed directly via it's Project DOI: 10.21228/M8J407 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001879
Study Title	Proteomics reveals an increase in the abundance of glycolytic and ethanolic fermentation enzymes in developing sugarcane culms during sucrose accumulation
Study Summary	Sugarcane is an economically important crop contributing to the world’s sugar and ethanol production with 80% and 40%, respectively. Metabolites from I5-4M and I9-4M were extracted from six biological samples of four-month-old plants. Following the removal of leaves, the internodes were identified and cut, and the bark removed, and the remaining tissue was immediately frozen in liquid nitrogen.
Institute	ESALQ-USP
Department	Genetics
Laboratory	Laboratório Max Feffer de Genética de Plantas
Last Name	Cataldi
First Name	Thais
Address	Padua Dias Avenue, 11, Piracicaba, São Paulo, 13418-900, Brazil
Email	thais.cataldi@usp.br
Phone	+551934294248
Submit Date	2021-07-19
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	GC-MS
Release Date	2022-02-16
Release Version	1

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Treatment:

Treatment ID:	TR001968
Treatment Summary:	Sugarcane cultivar SP80-3280 was grown in greenhouse conditions, at Piracicaba, São Paulo, Brazil (22°43’31’’S; 47°38’57’’W). Culms with one bud were planted in plastic trays and after acclimatization for two months, nine plants were transferred to 100 L plastic pots, containing soil (latosol). The temperature in the greenhouse was automatically adjusted to 28ºC ± 1ºC and 12/12 h light/dark photoperiod. Plants were watered to maintain soil capacity once a day, in the morning. Fertilizer was supplied once a week using a commercial fertilizer (Plant Prod®, N 15: P2O5 15: K2O 30, Plant Products CO. Canada) at the rate of 2 L per pot (4g/L). The replicates, distributed in a randomized design, were harvest at 4 month-old. During harvest, leaves were excised from the main culm and the internodes 5 and 9 from each plant, were separated and peeled from the top towards the base. Each individual internode was frozen in liquid nitrogen and kept at -80ºC. Six biological replicates from each internode were harvested.

Treatment ID:

TR001968

Treatment Summary:

Sugarcane cultivar SP80-3280 was grown in greenhouse conditions, at Piracicaba, São Paulo, Brazil (22°43’31’’S; 47°38’57’’W). Culms with one bud were planted in plastic trays and after acclimatization for two months, nine plants were transferred to 100 L plastic pots, containing soil (latosol). The temperature in the greenhouse was automatically adjusted to 28ºC ± 1ºC and 12/12 h light/dark photoperiod. Plants were watered to maintain soil capacity once a day, in the morning. Fertilizer was supplied once a week using a commercial fertilizer (Plant Prod®, N 15: P2O5 15: K2O 30, Plant Products CO. Canada) at the rate of 2 L per pot (4g/L). The replicates, distributed in a randomized design, were harvest at 4 month-old. During harvest, leaves were excised from the main culm and the internodes 5 and 9 from each plant, were separated and peeled from the top towards the base. Each individual internode was frozen in liquid nitrogen and kept at -80ºC. Six biological replicates from each internode were harvested.