Summary of Study ST001879

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001185. The data can be accessed directly via it's Project DOI: 10.21228/M8J407 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001879
Study TitleProteomics reveals an increase in the abundance of glycolytic and ethanolic fermentation enzymes in developing sugarcane culms during sucrose accumulation
Study SummarySugarcane is an economically important crop contributing to the world’s sugar and ethanol production with 80% and 40%, respectively. Metabolites from I5-4M and I9-4M were extracted from six biological samples of four-month-old plants. Following the removal of leaves, the internodes were identified and cut, and the bark removed, and the remaining tissue was immediately frozen in liquid nitrogen.
Institute
ESALQ-USP
DepartmentGenetics
LaboratoryLaboratório Max Feffer de Genética de Plantas
Last NameCataldi
First NameThais
AddressPadua Dias Avenue, 11, Piracicaba, São Paulo, 13418-900, Brazil
Emailthais.cataldi@usp.br
Phone+551934294248
Submit Date2021-07-19
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2022-02-16
Release Version1
Thais Cataldi Thais Cataldi
https://dx.doi.org/10.21228/M8J407
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003039
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Agilent DB5 (20m x 0.18 mm i.d. x 0.18 μm)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode UNSPECIFIED
Units relative intensity

MS:

MS ID:MS002829
Analysis ID:AN003039
Instrument Name:Leco Pegasus 4D GCxGC TOF
Instrument Type:GC x GC-TOF
MS Type:EI
MS Comments:The column effluent was introduced into the ion source of a GC×GC/TOF-MS (Pegasus 4D, Leco Corp., St. Joseph, USA). The transfer line and the ion source temperatures were 280 and 250°C, respectively. Ions were generated by a 70-eV electron beam at an ionization current of 2.0 mA, and 10 spectra s-1 were recorded in the mass range m/z 45–800. The ChromaTOF software v. 4.51 (Leco Corp., St. Joseph, USA) was used to perform baseline correction, deconvolution, peak detection, retention time alignment and library matching. NIST mass spectral library was used to metabolite identification. Multivariate (Partial least squares-discriminant analysis, PLS-DA) and univariate (t-test, FDR adjusted p ≤ 0.05) analysis were done in MetaboAnalyst 5.0 (Xia et al., 2015). To reduce systematic variance and to improve the performance for downstream statistical analysis data were log-transformed and auto-scaled prior to data analysis. Metabolites detected in at least three replicates in one group (I5 or I9) and not identified in any replicate of the other group were named “exclusive” metabolites.
Ion Mode:UNSPECIFIED
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