Summary of Study ST001879
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001185. The data can be accessed directly via it's Project DOI: 10.21228/M8J407 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001879 |
| Study Title | Proteomics reveals an increase in the abundance of glycolytic and ethanolic fermentation enzymes in developing sugarcane culms during sucrose accumulation |
| Study Summary | Sugarcane is an economically important crop contributing to the world’s sugar and ethanol production with 80% and 40%, respectively. Metabolites from I5-4M and I9-4M were extracted from six biological samples of four-month-old plants. Following the removal of leaves, the internodes were identified and cut, and the bark removed, and the remaining tissue was immediately frozen in liquid nitrogen. |
| Institute | ESALQ-USP |
| Department | Genetics |
| Laboratory | Laboratório Max Feffer de Genética de Plantas |
| Last Name | Cataldi |
| First Name | Thais |
| Address | Padua Dias Avenue, 11, Piracicaba, São Paulo, 13418-900, Brazil |
| thais.cataldi@usp.br | |
| Phone | +551934294248 |
| Submit Date | 2021-07-19 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2022-02-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003039 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Agilent DB5 (20m x 0.18 mm i.d. x 0.18 μm) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF |
| Ion Mode | UNSPECIFIED |
| Units | relative intensity |
MS:
| MS ID: | MS002829 |
| Analysis ID: | AN003039 |
| Instrument Name: | Leco Pegasus 4D GCxGC TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| MS Comments: | The column effluent was introduced into the ion source of a GC×GC/TOF-MS (Pegasus 4D, Leco Corp., St. Joseph, USA). The transfer line and the ion source temperatures were 280 and 250°C, respectively. Ions were generated by a 70-eV electron beam at an ionization current of 2.0 mA, and 10 spectra s-1 were recorded in the mass range m/z 45–800. The ChromaTOF software v. 4.51 (Leco Corp., St. Joseph, USA) was used to perform baseline correction, deconvolution, peak detection, retention time alignment and library matching. NIST mass spectral library was used to metabolite identification. Multivariate (Partial least squares-discriminant analysis, PLS-DA) and univariate (t-test, FDR adjusted p ≤ 0.05) analysis were done in MetaboAnalyst 5.0 (Xia et al., 2015). To reduce systematic variance and to improve the performance for downstream statistical analysis data were log-transformed and auto-scaled prior to data analysis. Metabolites detected in at least three replicates in one group (I5 or I9) and not identified in any replicate of the other group were named “exclusive” metabolites. |
| Ion Mode: | UNSPECIFIED |
