Summary of Study ST001951
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001238. The data can be accessed directly via it's Project DOI: 10.21228/M8PH6J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001951 |
| Study Title | Quantification of ω-3 fatty acids and their derivatives in lungs from hypoxia-induced pulmonary hypertension (PH) mice. |
| Study Summary | Using a liquid chromatography tandem mass spectrometry (LC-MS/MS) system, we quantified the ω-3 fatty acid (EPA and DHA) metabolites in the lung tissues of mice with PH induced by chronic hypoxia (10% oxygen concentration). |
| Institute | University of Tokyo |
| Last Name | Kono |
| First Name | Nozomu |
| Address | 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan |
| nozomu@mol.f.u-tokyo.ac.jp | |
| Phone | +81-3-5841-4723 |
| Submit Date | 2021-10-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-03-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003176 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu Nexera UC/s system (Shimadzu) |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Ion trap |
| MS instrument name | ABI Sciex 4500 Qtrap |
| Ion Mode | NEGATIVE |
| Units | ng/mg tissue |
Chromatography:
| Chromatography ID: | CH002348 |
| Instrument Name: | Shimadzu Nexera UC/s system (Shimadzu) |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | mobile phase A (water/acetic acid (100/0.1, v/v) containing 10 mM ammonium acetate) and mobile phase B (acetonitrile/methanol (4/1, v/v) containing 10 mM ammonium acetate) in a gradient programme (0–2 min: 90% A; 2–10 min: 90% A →30% A; 10–24 min: 30% A →27% A; 24–27 min: 1% A; 27–32 min: 90% A) with a flow rate of 0.2 mL/min(0–10 min), 0.1 mL/min(10–15 min),0.2 mL/min(15–24 min) and 0.5 mL/min(24–32 min). |
| Flow Rate: | (0–2 min: 90% A; 2–10 min: 90% A →30% A; 10–24 min: 30% A →27% A; 24–27 min: 1% A; 27–32 min: 90% A) with a flow rate of 0.2 mL/min(0–10 min), 0.1 mL/min(10–15 min),0.2 mL/min(15–24 min) and 0.5 mL/min(24–32 min) |
| Solvent A: | 100% water; 0.1% acetic acid; 10 mM ammonium acetate |
| Solvent B: | 80% acetonitrile/20% methanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
