Summary of Study ST001951

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001238. The data can be accessed directly via it's Project DOI: 10.21228/M8PH6J This work is supported by NIH grant, U2C- DK119886.

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Study IDST001951
Study TitleQuantification of ω-3 fatty acids and their derivatives in lungs from hypoxia-induced pulmonary hypertension (PH) mice.
Study SummaryUsing a liquid chromatography tandem mass spectrometry (LC-MS/MS) system, we quantified the ω-3 fatty acid (EPA and DHA) metabolites in the lung tissues of mice with PH induced by chronic hypoxia (10% oxygen concentration).
Institute
University of Tokyo
Last NameKono
First NameNozomu
Address7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Emailnozomu@mol.f.u-tokyo.ac.jp
Phone+81-3-5841-4723
Submit Date2021-10-17
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-03-31
Release Version1
Nozomu Kono Nozomu Kono
https://dx.doi.org/10.21228/M8PH6J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003176
Analysis type MS
Chromatography type Reversed phase
Chromatography system Shimadzu Nexera UC/s system (Shimadzu)
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 4500 Qtrap
Ion Mode NEGATIVE
Units ng/mg tissue

MS:

MS ID:MS002954
Analysis ID:AN003176
Instrument Name:ABI Sciex 4500 Qtrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:The instrument parameters of QTRAP4500 for negative ion mode were as follows: curtain gas, 10 psi; ionspray voltage, −4500 V; temperature, 600°C; ion source gas 1, 70 psi; ion source gas 2, 80 psi. and quantification was performed using MultiQuant™ software (SCIEX).
Ion Mode:NEGATIVE
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