Summary of Study ST002061
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001305. The data can be accessed directly via it's Project DOI: 10.21228/M81H6N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002061 |
| Study Title | Glutamine flux in macrophages treated with stable-isotope labeled analog 4 mM (U-13C5) glutamine |
| Study Type | Glutamine flux |
| Study Summary | 107 BMDMs per group (Sirt3 WT and Sirt3 K223R)were seeded in 10cm plates and incubated in RPMI-1640 cell culture medium with 10% FBS. Prior to isotopic labeling, the medium was replaced with RPMI-1640 without glutamine for 4 hrs. Then stable-isotope labeled analog 4 mM (U-13C5) glutamine (Cambridge Isotope) was added together with IL-4 (20ng/ml) for 4 hrs. |
| Institute | Shanghai Jiao Tong University affiliated Renji Hospital |
| Department | Department of Urology |
| Laboratory | Cheng Jinke's Lab |
| Last Name | Zhou |
| First Name | Wei |
| Address | N0.280 South Chongqing Road |
| joesphchou@alumni.sjtu.edu.cn | |
| Phone | +8615216716293 |
| Submit Date | 2022-01-19 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003360 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | ThermoFisher Ultimate 3000 UHPLC system |
| Column | Waters BEH Amide |
| MS Type | ESI |
| MS instrument type | Hybrid Quadrupole-Orbitrap™ |
| MS instrument name | ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE) |
| Ion Mode | NEGATIVE |
| Units | pmoles/I |
Chromatography:
| Chromatography ID: | CH002486 |
| Chromatography Summary: | Chromatographic separation was performed on a ThermoFisher Ultimate 3000 UHPLC system with a Waters BEH Amide column (2.1mm × 100 mm, 1.7 μm). The injection volume was 2μL and the flow rate was 0.25 mL/min. The column temperature was 15°C. The mobile phases consisted of water with 0.01% formic acid and 2 mM ammonium formate (phase A) and acetonitrile (phase B). A linear gradient elution was performed with the following program: 0 min, 90%B; 4 min, 85% B; 11 min, 75%B; 14 min, 70%B, 14.5min, 50%B and held to 17 min; 17.1 min, 90%B and held to 20.01 min. |
| Instrument Name: | ThermoFisher Ultimate 3000 UHPLC system |
| Column Name: | Waters BEH Amide |
| Column Temperature: | 15 |
| Flow Gradient: | 0 min, 90%B; 4 min, 85% B; 11 min, 75%B; 14 min, 70%B, 14.5min, 50%B and held to 17 min; 17.1 min, 90%B and held to 20.01 min. |
| Flow Rate: | 0.25ml/min |
| Solvent A: | 100% water; 0.01% formic acid; 2 mM ammonium formate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
