Summary of Study ST002061
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001305. The data can be accessed directly via it's Project DOI: 10.21228/M81H6N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002061 |
| Study Title | Glutamine flux in macrophages treated with stable-isotope labeled analog 4 mM (U-13C5) glutamine |
| Study Type | Glutamine flux |
| Study Summary | 107 BMDMs per group (Sirt3 WT and Sirt3 K223R)were seeded in 10cm plates and incubated in RPMI-1640 cell culture medium with 10% FBS. Prior to isotopic labeling, the medium was replaced with RPMI-1640 without glutamine for 4 hrs. Then stable-isotope labeled analog 4 mM (U-13C5) glutamine (Cambridge Isotope) was added together with IL-4 (20ng/ml) for 4 hrs. |
| Institute | Shanghai Jiao Tong University affiliated Renji Hospital |
| Department | Department of Urology |
| Laboratory | Cheng Jinke's Lab |
| Last Name | Zhou |
| First Name | Wei |
| Address | N0.280 South Chongqing Road |
| joesphchou@alumni.sjtu.edu.cn | |
| Phone | +8615216716293 |
| Submit Date | 2022-01-19 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-02-07 |
| Release Version | 1 |
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Project:
| Project ID: | PR001305 |
| Project DOI: | doi: 10.21228/M81H6N |
| Project Title: | Glutamine Flux analysis in Macrophages |
| Project Type: | MS quantitive analysis |
| Project Summary: | To determine the effect of Sirt3 K223R on glutaminolysis, we traced glutamine metabolic influx in macrophages. The data demonstrated that Sirt3 K223R did not alter glutamine uptake and glutamate production in BMDMs after IL-4 treatment . However, 5C(M+5) labeled-αKG showed a higher ratio in IL-4-treated Sirt3 KR macrophages than that in IL-4-treated Sirt3 WT macrophages, suggesting that SENP1-Sirt3 axis mainly involves the conversion of glutamate to αKG of glutaminolysis in macrophage M2 polarization. |
| Institute: | Shanghai Jiao Tong University affiliated Renji Hospital |
| Department: | Department of Urology |
| Laboratory: | Cheng Jinke's Lab |
| Last Name: | Zhou |
| First Name: | Wei |
| Address: | N0.280 South Chongqing Road |
| Email: | joesphchou@alumni.sjtu.edu.cn |
| Phone: | +8615216716293 |
| Funding Source: | National Natural Science Foundation of China |
| Project Comments: | In summary, we reveal that SENP1-Sirt3 signaling plays a crucial role in promoting αKG production and M2 polarization. |
| Publications: | Cell Reports |
| Contributors: | Wei Zhou |
