Summary of Study ST002061

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001305. The data can be accessed directly via it's Project DOI: 10.21228/M81H6N This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002061
Study TitleGlutamine flux in macrophages treated with stable-isotope labeled analog 4 mM (U-13C5) glutamine
Study TypeGlutamine flux
Study Summary107 BMDMs per group (Sirt3 WT and Sirt3 K223R)were seeded in 10cm plates and incubated in RPMI-1640 cell culture medium with 10% FBS. Prior to isotopic labeling, the medium was replaced with RPMI-1640 without glutamine for 4 hrs. Then stable-isotope labeled analog 4 mM (U-13C5) glutamine (Cambridge Isotope) was added together with IL-4 (20ng/ml) for 4 hrs.
Institute
Shanghai Jiao Tong University affiliated Renji Hospital
DepartmentDepartment of Urology
LaboratoryCheng Jinke's Lab
Last NameZhou
First NameWei
AddressN0.280 South Chongqing Road
Emailjoesphchou@alumni.sjtu.edu.cn
Phone+8615216716293
Submit Date2022-01-19
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-02-07
Release Version1
Wei Zhou Wei Zhou
https://dx.doi.org/10.21228/M81H6N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003360
Analysis type MS
Chromatography type HILIC
Chromatography system ThermoFisher Ultimate 3000 UHPLC system
Column Waters BEH Amide
MS Type ESI
MS instrument type Hybrid Quadrupole-Orbitrap™
MS instrument name ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE)
Ion Mode NEGATIVE
Units pmoles/I

MS:

MS ID:MS003129
Analysis ID:AN003360
Instrument Name:ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE)
Instrument Type:Hybrid Quadrupole-Orbitrap™
MS Type:ESI
MS Comments:The eluents were analyzed on a ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE) in Heated Electrospray Ionization Negative (HESI-) mode, separately. Spray voltage was set to 4000 V. Capillary and Probe Heater Temperature were separately 320 °C and 320 °C. Sheath gas flow rate was 35 (Arb, arbitrary unit), and Aux gas flow rate was 10 (Arb). S-Lens RF Level was 50 (Arb). The full scan was operated at a high-resolution of 70000 FWHM (m/z=200) at a range of 70- 1050 m/z with AGC Target setting at 3×106.
Ion Mode:NEGATIVE
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