Summary of Study ST002069
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001311. The data can be accessed directly via it's Project DOI: 10.21228/M88113 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002069 |
| Study Title | Systemic impact of the expression of the mitochondrial alternative oxidase on Drosophila development |
| Study Summary | Despite the beneficial effects shown when the mitochondrial alternative oxidase AOX from Ciona intestinalis (Tunicata: Ascidiacea) is xenotopically expressed in mammalian and insect models, important detrimental outcomes have also been reported, raising concerns regarding its envisioned deployment as a therapy enzyme for human mitochondrial and related diseases. We have shown previously that AOX-expressing flies present a dramatic drop in pupal viability when the larvae are cultured on a low nutrient (LN) diet, indicating that AOX interferes with normal developmental metabolism. Here, we applied the metabolomics approach to gain insights into the molecular basis of the fatal developmental interaction between AOX expression and LN diet. We investigated the whole-body metabolome of AOX-expressing and control larvae cultured on SD and LN diets. |
| Institute | Tampere University |
| Department | Faculty of Medicine and Health Technology |
| Last Name | Dufour |
| First Name | Eric |
| Address | Arvo Ylpön katu 34, 33520 Tampere, Finlândia |
| eric.dufour@tuni.fi | |
| Phone | +358 (0)50 413 0493 |
| Submit Date | 2022-01-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-01-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003378 | AN003379 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | UltiMate 3000RSLC HPLC (Thermo Scientific) | UltiMate 3000RSLC HPLC (Thermo Scientific) |
| Column | Phenomenex Luna NH2 (150 x 2mm) | Phenomenex Luna NH2 (150 x 2mm) |
| MS Type | ESI | ESI |
| MS instrument type | Ion trap | Ion trap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | intensity (area under the curve) | intensity (area under the curve) |
Chromatography:
| Chromatography ID: | CH002497 |
| Chromatography Summary: | Separation was achieved using a linear gradient from 15% to 90% A over 18 min. This was followed by an isocratic step at 90% A for 9 min and re-equilibration to the initial 15% A for 7 min. |
| Instrument Name: | UltiMate 3000RSLC HPLC (Thermo Scientific) |
| Column Name: | Phenomenex Luna NH2 (150 x 2mm) |
| Column Temperature: | 27 C |
| Flow Rate: | 200 ul/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
