Summary of Study ST002069

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001311. The data can be accessed directly via it's Project DOI: 10.21228/M88113 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002069
Study Title	Systemic impact of the expression of the mitochondrial alternative oxidase on Drosophila development
Study Summary	Despite the beneficial effects shown when the mitochondrial alternative oxidase AOX from Ciona intestinalis (Tunicata: Ascidiacea) is xenotopically expressed in mammalian and insect models, important detrimental outcomes have also been reported, raising concerns regarding its envisioned deployment as a therapy enzyme for human mitochondrial and related diseases. We have shown previously that AOX-expressing flies present a dramatic drop in pupal viability when the larvae are cultured on a low nutrient (LN) diet, indicating that AOX interferes with normal developmental metabolism. Here, we applied the metabolomics approach to gain insights into the molecular basis of the fatal developmental interaction between AOX expression and LN diet. We investigated the whole-body metabolome of AOX-expressing and control larvae cultured on SD and LN diets.
Institute	Tampere University
Department	Faculty of Medicine and Health Technology
Last Name	Dufour
First Name	Eric
Address	Arvo Ylpön katu 34, 33520 Tampere, Finlândia
Email	eric.dufour@tuni.fi
Phone	+358 (0)50 413 0493
Submit Date	2022-01-29
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2023-01-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002161
Sampleprep Summary:	: Frozen larvae were homogenized in 1 ml cold (-80 °C) 80% methanol on dry ice using a probe homogenizer. Samples were clarified by centrifugation for 5 min at 16,000 x gmax at 4 °C and the supernatants were transferred to new Eppendorf tubes. The remaining pellets were resuspended in RIPA lysis buffer and total protein content was determined by the BCA method. A volume of the supernatant equivalent to 5 µg of protein was transferred to a glass vial, dried using an EZ-2 Elite evaporator (SP Scientific), and stored at -80 °C until analysis by LC-MS.

Sampleprep ID:

SP002161

Sampleprep Summary:

: Frozen larvae were homogenized in 1 ml cold (-80 °C) 80% methanol on dry ice using a probe homogenizer. Samples were clarified by centrifugation for 5 min at 16,000 x gmax at 4 °C and the supernatants were transferred to new Eppendorf tubes. The remaining pellets were resuspended in RIPA lysis buffer and total protein content was determined by the BCA method. A volume of the supernatant equivalent to 5 µg of protein was transferred to a glass vial, dried using an EZ-2 Elite evaporator (SP Scientific), and stored at -80 °C until analysis by LC-MS.