Summary of Study ST002272
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001453. The data can be accessed directly via it's Project DOI: 10.21228/M8X13R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002272 |
| Study Title | Metabolic changes in seeds of malting barley produced under drought or elevated temperature |
| Study Type | Barley seed phenotyping and GC-MS based metabolomic analysis |
| Study Summary | Plants of a “Hana-type” landrace (B1) were taller, flowered earlier and produced heavier, larger and more vigorous seeds that resisted ageing longer compared to a semi-dwarf breeding line (B2). Drought significantly reduced seed yield in both genotypes, and elevated temperature reduced seed size. Genotype B2 showed partial thermodormancy that was alleviated by drought and elevated temperature, in line with lower abundance of the TF ABI5, a key regulator of seed dormancy and vigour. Metabolite profiling revealed clear differences between the embryos of B1 and B2. Drought, but not elevated temperature, affected the metabolism of amino acids, organic acids, osmolytes and nitrogen assimilation, in the seeds of both genotypes. |
| Institute | INRAE |
| Last Name | CLEMENT |
| First Name | Gilles |
| Address | Route de ST-Cyr, Versailles, Ile de France, 78026, France |
| gilles.clement@inrae.fr | |
| Phone | +33 (0) 1 30 83 31 67 |
| Submit Date | 2022-08-29 |
| Num Groups | 6 |
| Total Subjects | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2023-03-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003714 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rxi-5Sil (30m x 0.25mm,0.25m) with 10m precolumn |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975C |
| Ion Mode | POSITIVE |
| Units | nmoles/mg DW and arbitrary/mg DW |
Chromatography:
| Chromatography ID: | CH002751 |
| Chromatography Summary: | The instrument was an Agilent 7890A gas chromatograph coupled to an Agilent 5975C mass spectrometer. The column was an Rxi-5SilMS from Restek (30 m with 10 m integraguard column). The liner (Restek # 20994) was changed before each derivatization series. Oven temperature ramp was 70 °C for 7 min then 10 °C/min to 330 °C for 5 min (run length 38 min). Helium constant flow was 0.7 mL/min. Temperatures were the following: injector: 250°C, transfer line: 290°C, source: 250 °C and quadripole 150 °C. 5 scans per second were acquired spanning a 50 to 600 Da range. Instrument was tuned with PFTBA with the 69 m/z and 219 m/z of equal intensities. 5 scans per second were acquired. The split mode conditions were: 70°C for 2 min then 30°C per min to 330 °C for 5 min. Helium constant flow 1 mL/min. |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rxi-5Sil (30m x 0.25mm,0.25m) with 10m precolumn |
| Flow Rate: | 0.7 ml/min |
| Injection Temperature: | 250°C |
| Chromatography Type: | GC |
