Summary of Study ST002272
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001453. The data can be accessed directly via it's Project DOI: 10.21228/M8X13R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002272 |
Study Title | Metabolic changes in seeds of malting barley produced under drought or elevated temperature |
Study Type | Barley seed phenotyping and GC-MS based metabolomic analysis |
Study Summary | Plants of a “Hana-type” landrace (B1) were taller, flowered earlier and produced heavier, larger and more vigorous seeds that resisted ageing longer compared to a semi-dwarf breeding line (B2). Drought significantly reduced seed yield in both genotypes, and elevated temperature reduced seed size. Genotype B2 showed partial thermodormancy that was alleviated by drought and elevated temperature, in line with lower abundance of the TF ABI5, a key regulator of seed dormancy and vigour. Metabolite profiling revealed clear differences between the embryos of B1 and B2. Drought, but not elevated temperature, affected the metabolism of amino acids, organic acids, osmolytes and nitrogen assimilation, in the seeds of both genotypes. |
Institute | INRAE |
Last Name | CLEMENT |
First Name | Gilles |
Address | Route de ST-Cyr, Versailles, Ile de France, 78026, France |
gilles.clement@inrae.fr | |
Phone | +33 (0) 1 30 83 31 67 |
Submit Date | 2022-08-29 |
Num Groups | 6 |
Total Subjects | 18 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2023-03-01 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002370 |
Treatment Summary: | Two barley genotypes (Hordeum vulgare L.), the Austrian landrace HOR 2110 (termed “B1”) and the breeding line HOR 4710 (termed “B2”) were used. For each genotype, seedlings were grown in a greenhouse in pots (30 x 30 x 15 cm, 4 plants per pot) at a 23/15 °C day/night cycle until anthesis. After half of the spikes had flowered, plants were kept for another seven days under the same conditions, and then randomly selected and subjected either to control conditions (C, 22/18 °C and regular watering) or elevated temperature (ET, 28/25 °C and regular watering,) or drought (D, 22/18 °C and 15 % field capacity). After harvest, seeds were cleaned, dried at 20 °C and 20 % relative humidity (RH) for eight weeks (for after-ripening), and then stored at 18 °C. |