Summary of Study ST002272
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001453. The data can be accessed directly via it's Project DOI: 10.21228/M8X13R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002272 |
| Study Title | Metabolic changes in seeds of malting barley produced under drought or elevated temperature |
| Study Type | Barley seed phenotyping and GC-MS based metabolomic analysis |
| Study Summary | Plants of a “Hana-type” landrace (B1) were taller, flowered earlier and produced heavier, larger and more vigorous seeds that resisted ageing longer compared to a semi-dwarf breeding line (B2). Drought significantly reduced seed yield in both genotypes, and elevated temperature reduced seed size. Genotype B2 showed partial thermodormancy that was alleviated by drought and elevated temperature, in line with lower abundance of the TF ABI5, a key regulator of seed dormancy and vigour. Metabolite profiling revealed clear differences between the embryos of B1 and B2. Drought, but not elevated temperature, affected the metabolism of amino acids, organic acids, osmolytes and nitrogen assimilation, in the seeds of both genotypes. |
| Institute | INRAE |
| Last Name | CLEMENT |
| First Name | Gilles |
| Address | Route de ST-Cyr, Versailles, Ile de France, 78026, France |
| gilles.clement@inrae.fr | |
| Phone | +33 (0) 1 30 83 31 67 |
| Submit Date | 2022-08-29 |
| Num Groups | 6 |
| Total Subjects | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2023-03-01 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002364 |
| Sampleprep Summary: | For metabolite profiling, three biological replicates of 12 mg of barley embryos manually dissected from dry mature seeds were ground with mortar and pestle in liquid nitrogen and stored at -80 °C. All steps were performed in 2 ml Safelock Eppendorf tubes. The ground frozen samples (12 mg) were resuspended in 1 ml of frozen (-20°C) Water:Acetonitrile:Isopropanol (2:3:3 v/v/v/) containing Ribitol at 4 mg.L-1 and extracted for 10 min at 4°C with shaking at 1400 rpm in an Eppendorf Thermomixer. Insoluble material was removed by centrifugation at 20000g for 5 min. 25 µL were collected and dried for 150 min in a SpeedVac. Fiehn et al (the Plant Journal (2008) 53, 691-704). |
| Processing Storage Conditions: | -20℃ |
| Extract Storage: | -80℃ |
