Summary of Study ST002494
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001610. The data can be accessed directly via it's Project DOI: 10.21228/M8KT5B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002494 |
| Study Title | Disrupted intestinal microbiota contributes to the pathogenesis of anorexia nervosa (Part 1) |
| Study Summary | Anorexia nervosa (AN) is an eating disorder with a high mortality affecting about 1% of women, where no evidence-based effective treatment exists. The pathogenesis likely involves genetic and environmental alterations. We hypothesized that a disrupted gut microbiota contributes to AN pathogenesis. In analyses comparing 70 AN with 77 healthy females, we found multiple taxa, functional modules, structural variants and growth rates of bacterial gut microbiota, and viral gut microbiota that were altered in AN with parts of these perturbations linked to estimates of eating behavior and mental health. In silico, causal inference analyses implied serum bacterial metabolites mediated parts of the impact of altered gut microbiota on AN behavior, and in vivo, three independent fecal microbiota transplantation from AN cases to germ-free mice under energy restricted feeding mirroring AN eating behavior consistently induced a lower body weight gain and hypothalamic and adipose tissue gene expressions related to aberrant energy metabolism and eating and mental behavior. |
| Institute | Örebro University |
| Last Name | McGlinchey |
| First Name | Aidan |
| Address | Room 2217, Södra Grev Rosengatan 30, 70362 Örebro |
| aidan.mcglinchey@oru.se | |
| Phone | +46 0736485638 |
| Submit Date | 2022-05-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | GC-MS |
| Release Date | 2023-02-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004092 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 7890B |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | EI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 7200 QTOF |
| Ion Mode | NEGATIVE |
| Units | Raw output from software |
Chromatography:
| Chromatography ID: | CH003030 |
| Chromatography Summary: | The plate was preconditioned with 450 μL acetonitrile before the addition of 100 μL of sample and 10 μL of PFAS and BA internal standard mixture (200 ng/mL and 1000 ng/mL respectively). Thereafter, 450 μL of acetonitrile containing 1% formic acid were added to each well and the samples extracted using a 10” vacuum manifold. The eluate evaporated to dryness under nitrogen gas flow and reconstituted to 80 μL of MeOH/2 mM aqueous NH4AC. Chromatographic separation was carried out using an Acquity UPLC BEH C18 column (100 mm × 2.1 mm i.d., 1.7 μm particle size), fitted with a C18 precolumn (Waters Corporation, Wexford, Ireland). Mobile phase A consisted of H2O:MeOH (v/v 70:30) and mobile phase B of MeOH with both phases containing 2mM ammonium acetate as an ionization agent. The flow rate was set at 0.4 mLmin-1 with the elution gradient as follows: 0-1.5 min, mobile phase B was increased from 5% to 30%; 1.5-4.5 min, mobile phase B increased to 70%; 4.5-7.5 min, mobile phase B increased to 100% and held for 5.5 min. A post-time of 5 min was used to regain the initial conditions for the next analysis. The total run time per sample was 18 min. The dual ESI ionization source was settings were as follows: capillary voltage was 4.5 kV, nozzle voltage 1500 V, N2 pressure in the nebulized was 21 psi and the N2 flow rate and temperature as sheath gas was 11 L min-1 and 379 °C, respectively. |
| Instrument Name: | Agilent 7890B |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 70 |
| Flow Gradient: | The elution gradient was as follows: 0-1.5 min, mobile phase B was increased from 5% to 30%; 1.5-4.5 min, mobile phase B increased to 70%; 4.5-7.5 min, mobile phase B increased to 100% and held for 5.5 min. A post-time of 5 min was used to regain the initial conditions for the next analysis. The total run time per sample was 18 min. |
| Flow Rate: | 0.4ml/min |
| Solvent A: | 70% water/30% methanol |
| Solvent B: | 100% methanol |
| Chromatography Type: | Reversed phase |
