Summary of Study ST002494
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001610. The data can be accessed directly via it's Project DOI: 10.21228/M8KT5B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002494 |
| Study Title | Disrupted intestinal microbiota contributes to the pathogenesis of anorexia nervosa (Part 1) |
| Study Summary | Anorexia nervosa (AN) is an eating disorder with a high mortality affecting about 1% of women, where no evidence-based effective treatment exists. The pathogenesis likely involves genetic and environmental alterations. We hypothesized that a disrupted gut microbiota contributes to AN pathogenesis. In analyses comparing 70 AN with 77 healthy females, we found multiple taxa, functional modules, structural variants and growth rates of bacterial gut microbiota, and viral gut microbiota that were altered in AN with parts of these perturbations linked to estimates of eating behavior and mental health. In silico, causal inference analyses implied serum bacterial metabolites mediated parts of the impact of altered gut microbiota on AN behavior, and in vivo, three independent fecal microbiota transplantation from AN cases to germ-free mice under energy restricted feeding mirroring AN eating behavior consistently induced a lower body weight gain and hypothalamic and adipose tissue gene expressions related to aberrant energy metabolism and eating and mental behavior. |
| Institute | Örebro University |
| Last Name | McGlinchey |
| First Name | Aidan |
| Address | Room 2217, Södra Grev Rosengatan 30, 70362 Örebro |
| aidan.mcglinchey@oru.se | |
| Phone | +46 0736485638 |
| Submit Date | 2022-05-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | GC-MS |
| Release Date | 2023-02-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004092 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 7890B |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | EI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 7200 QTOF |
| Ion Mode | NEGATIVE |
| Units | Raw output from software |
MS:
| MS ID: | MS003839 |
| Analysis ID: | AN004092 |
| Instrument Name: | Agilent 7200 QTOF |
| Instrument Type: | QTOF |
| MS Type: | EI |
| MS Comments: | MassHunter B.06.01 software (Agilent Technologies, Santa Clara, CA, USA) was used for all data acquisition. Identification of compounds was done by in-house spectral library using MS (and retention time), MS/MS information. Quantitation was based on a matrix matched calibration curve spiked with native compounds. The calibration curve consisted of concentrations ranging from 0 – 1600 ng mL -1 for BAs. The RSD for the BAs was on average 17.8 % for the QC samples and 19.4 % for the NIST samples. |
| Ion Mode: | NEGATIVE |
