Summary of Study ST002499

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001272. The data can be accessed directly via it's Project DOI: 10.21228/M89402 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002499
Study TitleMetabolomics analysis of stress erythroid progenitors (Part 2)
Study SummaryA time course study to assess the intracellular metabolic changes of splenic Kit+ stress erythroid progenitors
Pennsylvania State University
DepartmentVeterinary and Biomedical Sciences
LaboratoryPaulson Lab
Last NameRuan
First NameBaiye
Address228 AVBS Building Shortlidge Road University Park, PA 16802
Submit Date2023-02-23
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-03-22
Release Version1
Baiye Ruan Baiye Ruan application/zip

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Combined analysis:

Analysis ID AN004106
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Waters XBridge C18 (50 x 2.1mm,3um)
MS instrument type Orbitrap
MS instrument name Thermo Exactive Plus Orbitrap
Units peak area normalized to IS and cell number


Chromatography ID:CH003041
Chromatography Summary:The sample run order was randomized to reduce bias from instrument drift. 10 µl sample was subjected to LC-MS analysis on a Exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific) coupled to an Ultimate 3000 UHPLC system (Thermo Fisher Scientific). Reversed-phase chromatography mode was used to separate compounds on a Xselect C18 HSS column (Waters) with solvent A (97:3 water:methanol (v/v), 10 mM tributylamine, and 15 mM acetic acid ) and solvent B (methanol). The flow rate was 200 µl/min, and the total run time was 25 min. The gradient was 0 min, 0% B; 5 min, 20% B; 7.5 min, 55% B; 15 min, 65% B; 17.5 min, 95% B; and 21 min, 0% B. The mass spectrometer was operated in a negative-ion mode at a resolution of 140,000 at m/z 200 and with a scan range of 85 to 1000 m/z.
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters XBridge C18 (50 x 2.1mm,3um)
Column Temperature:standard
Flow Gradient:0 min, 0% B; 5 min, 20% B; 7.5 min, 55% B; 15 min, 65% B; 17.5 min, 95% B; and 21 min, 0% B.
Flow Rate:200 µl/min
Solvent A:97:3 water:methanol (v/v), 10 mM tributylamine, and 15 mM acetic acid
Solvent B:methanol
Chromatography Type:Reversed phase