Summary of Study ST002499
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001272. The data can be accessed directly via it's Project DOI: 10.21228/M89402 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002499 |
Study Title | Metabolomics analysis of stress erythroid progenitors (Part 2) |
Study Summary | A time course study to assess the intracellular metabolic changes of splenic Kit+ stress erythroid progenitors |
Institute | Pennsylvania State University |
Department | Veterinary and Biomedical Sciences |
Laboratory | Paulson Lab |
Last Name | Ruan |
First Name | Baiye |
Address | 228 AVBS Building Shortlidge Road University Park, PA 16802 |
bur27@psu.edu | |
Phone | 814-863-6306 |
Submit Date | 2023-02-23 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-03-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004106 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Waters XBridge C18 (50 x 2.1mm,3um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Exactive Plus Orbitrap |
Ion Mode | NEGATIVE |
Units | peak area normalized to IS and cell number |
Chromatography:
Chromatography ID: | CH003041 |
Chromatography Summary: | The sample run order was randomized to reduce bias from instrument drift. 10 µl sample was subjected to LC-MS analysis on a Exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific) coupled to an Ultimate 3000 UHPLC system (Thermo Fisher Scientific). Reversed-phase chromatography mode was used to separate compounds on a Xselect C18 HSS column (Waters) with solvent A (97:3 water:methanol (v/v), 10 mM tributylamine, and 15 mM acetic acid ) and solvent B (methanol). The flow rate was 200 µl/min, and the total run time was 25 min. The gradient was 0 min, 0% B; 5 min, 20% B; 7.5 min, 55% B; 15 min, 65% B; 17.5 min, 95% B; and 21 min, 0% B. The mass spectrometer was operated in a negative-ion mode at a resolution of 140,000 at m/z 200 and with a scan range of 85 to 1000 m/z. |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters XBridge C18 (50 x 2.1mm,3um) |
Column Temperature: | standard |
Flow Gradient: | 0 min, 0% B; 5 min, 20% B; 7.5 min, 55% B; 15 min, 65% B; 17.5 min, 95% B; and 21 min, 0% B. |
Flow Rate: | 200 µl/min |
Solvent A: | 97:3 water:methanol (v/v), 10 mM tributylamine, and 15 mM acetic acid |
Solvent B: | methanol |
Chromatography Type: | Reversed phase |