Summary of Study ST002499

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001272. The data can be accessed directly via it's Project DOI: 10.21228/M89402 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002499
Study TitleMetabolomics analysis of stress erythroid progenitors (Part 2)
Study SummaryA time course study to assess the intracellular metabolic changes of splenic Kit+ stress erythroid progenitors
Institute
Pennsylvania State University
DepartmentVeterinary and Biomedical Sciences
LaboratoryPaulson Lab
Last NameRuan
First NameBaiye
Address228 AVBS Building Shortlidge Road University Park, PA 16802
Emailbur27@psu.edu
Phone814-863-6306
Submit Date2023-02-23
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-03-22
Release Version1
Baiye Ruan Baiye Ruan
https://dx.doi.org/10.21228/M89402
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002589
Collection Summary:Murine spleens were harvested and disassociated to single cell suspension at a concentration of 1 x 10^8 cells/mL. Labeling reagent was added at a concentation of 50 μL/mL of sample. Cells were mixed and incubated at RT for 15 mins. Selection cocktail was added to sample at a 70 μL/mL of sample. Cells were mixed and incubated at RT for 15 mins. RapidSpheres were added to sample at a 50 μL/mL of sample. Cells were mixed and incubated at RT for 10 mins. Cells were washed with FACS buffer for 4 times, and were resuspended for downstream metabolomics analysis.
Sample Type:Stem cells
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