Summary of Study ST002813
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001760. The data can be accessed directly via it's Project DOI: 10.21228/M86T5X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002813 |
| Study Title | Effects of acute cold exposure on mouse metabolome |
| Study Summary | Cold-induced thermogenesis is widely studied as a potential avenue to treat obesity, but a thorough understanding of the metabolic changes driving thermogenesis is lacking. We analyzed mouse plasma and tissue samples from fasted mice housed at room temperature or acutely exposed to 4°C. First, C57BL/6 mice (n=41) were divided into 2 groups (n=20-21 per group) and underwent surgery to implant an indwelling arterial catheter. Mice originally housed at room temperature were housed without food for 6 hours at room temperature or 4°C, after which blood was collected from the arterial catheter and centrifuged, and plasma was collected for metabolomic analyses. Second, C57BL/6 mice (n=12) were divided into 2 groups (n=6 per group) and housed without food for 6 hours at room temperature or 4°C. Mice were euthanized, and tissues (brown adipose tissue, heart, liver, quadricepts muscle, diaphragm, and gonadal-white adipose tissue) were collected, freeze-clamped in liquid nitrogen, and used for metabolomic analyses. Overall our data detail a coordinated and broad metabolic response governing the thermogenic response to acute cold exposure in mice. |
| Institute | University of Pennsylvania |
| Department | Medicine |
| Laboratory | Arany Lab |
| Last Name | Bornstein |
| First Name | Marc |
| Address | 3400 Civic Center Blvd (Smilow 11-187) |
| marc.bornstein@pennmedicine.upenn.edu | |
| Phone | 7742796684 |
| Submit Date | 2023-08-09 |
| Num Groups | 4 |
| Total Subjects | 53 |
| Num Males | 53 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004575 | AN004576 | AN004577 | AN004578 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Waters XBridge BEH Amide (150 x 2.1mm,2.5um) | Waters XBridge BEH Amide (150 x 2.1mm,2.5um) | Agilent InfinityLab Poroshell 120 EC-C8 (150 x 2.1mm,2.7um) | Agilent InfinityLab Poroshell 120 EC-C8 (150 x 2.1mm,2.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Intensity | Intensity | Intensity | intensity |
Chromatography:
| Chromatography ID: | CH003439 |
| Chromatography Summary: | Chromatography for hydrophilic compounds |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters XBridge BEH Amide (150 x 2.1mm,2.5um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0 min, 85% B; 2 min, 85% B; 3 min, 80% B; 5 min, 80% B; 6 min, 75% B; 7 min, 75% B; 8 min, 70% B; 9 min, 70% B; 10 min, 50% B; 12 min, 50% B; 13 min, 25% B; 16 min, 25% B; 18 min, 0% B; 23 min, 0% B; 24 min, 85% B; and 30 min, 85% B. |
| Flow Rate: | 150 uL/min |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium acetate; mM ammonium hydroxide, pH 9.45) |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003440 |
| Chromatography Summary: | Chromatography for lipids |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C8 (150 x 2.1mm,2.7um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0 minutes, 25% B; 2 minutes, 25% B; 4 minutes, 65% B; 16 minutes, 100% B; 20 minutes, 100% B; 21 minutes, 25% B; 27 minutes, 25% B |
| Flow Rate: | 150 uL/min |
| Solvent A: | 90% water/10% methanol; 1mM ammonium acetate ; 0.2% acetic acid |
| Solvent B: | 2% methanol/98% isopropano; 1 mM ammonium acetate; 0.2% acetic acid |
| Chromatography Type: | Reversed phase |
