Summary of Study ST002813
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001760. The data can be accessed directly via it's Project DOI: 10.21228/M86T5X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002813 |
| Study Title | Effects of acute cold exposure on mouse metabolome |
| Study Summary | Cold-induced thermogenesis is widely studied as a potential avenue to treat obesity, but a thorough understanding of the metabolic changes driving thermogenesis is lacking. We analyzed mouse plasma and tissue samples from fasted mice housed at room temperature or acutely exposed to 4°C. First, C57BL/6 mice (n=41) were divided into 2 groups (n=20-21 per group) and underwent surgery to implant an indwelling arterial catheter. Mice originally housed at room temperature were housed without food for 6 hours at room temperature or 4°C, after which blood was collected from the arterial catheter and centrifuged, and plasma was collected for metabolomic analyses. Second, C57BL/6 mice (n=12) were divided into 2 groups (n=6 per group) and housed without food for 6 hours at room temperature or 4°C. Mice were euthanized, and tissues (brown adipose tissue, heart, liver, quadricepts muscle, diaphragm, and gonadal-white adipose tissue) were collected, freeze-clamped in liquid nitrogen, and used for metabolomic analyses. Overall our data detail a coordinated and broad metabolic response governing the thermogenic response to acute cold exposure in mice. |
| Institute | University of Pennsylvania |
| Department | Medicine |
| Laboratory | Arany Lab |
| Last Name | Bornstein |
| First Name | Marc |
| Address | 3400 Civic Center Blvd (Smilow 11-187) |
| marc.bornstein@pennmedicine.upenn.edu | |
| Phone | 7742796684 |
| Submit Date | 2023-08-09 |
| Num Groups | 4 |
| Total Subjects | 53 |
| Num Males | 53 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Temperature |
|---|---|---|
| SA301752 | BAT2 | 4°C |
| SA301753 | neg42 | 4°C |
| SA301754 | Diaphragm8 | 4°C |
| SA301755 | posi2 | 4°C |
| SA301756 | posi-40 | 4°C |
| SA301757 | BAT4 | 4°C |
| SA301758 | posi-42 | 4°C |
| SA301759 | neg40 | 4°C |
| SA301760 | neg38 | 4°C |
| SA301761 | BAT12 | 4°C |
| SA301762 | BAT10 | 4°C |
| SA301763 | Diaphragm6 | 4°C |
| SA301764 | neg4 | 4°C |
| SA301765 | BAT8 | 4°C |
| SA301766 | BAT6 | 4°C |
| SA301767 | posi-38 | 4°C |
| SA301768 | posi8 | 4°C |
| SA301769 | posi-30 | 4°C |
| SA301770 | posi-28 | 4°C |
| SA301771 | posi-26 | 4°C |
| SA301772 | posi-24 | 4°C |
| SA301773 | posi10 | 4°C |
| SA301774 | Diaphragm10 | 4°C |
| SA301775 | posi-32 | 4°C |
| SA301776 | posi-36 | 4°C |
| SA301777 | posi4 | 4°C |
| SA301778 | neg14 | 4°C |
| SA301779 | posi-34 | 4°C |
| SA301780 | posi6 | 4°C |
| SA301781 | Liver2 | 4°C |
| SA301782 | neg36 | 4°C |
| SA301783 | Quad12 | 4°C |
| SA301784 | neg20 | 4°C |
| SA301785 | neg26 | 4°C |
| SA301786 | Quad10 | 4°C |
| SA301787 | Quad8 | 4°C |
| SA301788 | Heart12 | 4°C |
| SA301789 | Heart2 | 4°C |
| SA301790 | neg24 | 4°C |
| SA301791 | neg22 | 4°C |
| SA301792 | Heart8 | 4°C |
| SA301793 | Heart6 | 4°C |
| SA301794 | Heart10 | 4°C |
| SA301795 | Heart4 | 4°C |
| SA301796 | neg28 | 4°C |
| SA301797 | Quad6 | 4°C |
| SA301798 | Liver8 | 4°C |
| SA301799 | neg18 | 4°C |
| SA301800 | neg34 | 4°C |
| SA301801 | Liver6 | 4°C |
| SA301802 | Liver4 | 4°C |
| SA301803 | Diaphragm4 | 4°C |
| SA301804 | Liver10 | 4°C |
| SA301805 | neg32 | 4°C |
| SA301806 | neg30 | 4°C |
| SA301807 | Quad4 | 4°C |
| SA301808 | Quad2 | 4°C |
| SA301809 | Diaphragm2 | 4°C |
| SA301810 | Liver12 | 4°C |
| SA301811 | posi-22 | 4°C |
| SA301812 | Diaphragm12 | 4°C |
| SA301813 | posi32 | 4°C |
| SA301814 | neg-26 | 4°C |
| SA301815 | neg-24 | 4°C |
| SA301816 | neg-22 | 4°C |
| SA301817 | neg-20 | 4°C |
| SA301818 | posi34 | 4°C |
| SA301819 | gWAT10 | 4°C |
| SA301820 | neg-28 | 4°C |
| SA301821 | neg-32 | 4°C |
| SA301822 | posi28 | 4°C |
| SA301823 | neg8 | 4°C |
| SA301824 | neg-30 | 4°C |
| SA301825 | posi30 | 4°C |
| SA301826 | gWAT12 | 4°C |
| SA301827 | neg-18 | 4°C |
| SA301828 | neg-8 | 4°C |
| SA301829 | posi40 | 4°C |
| SA301830 | neg-6 | 4°C |
| SA301831 | posi42 | 4°C |
| SA301832 | neg-2 | 4°C |
| SA301833 | neg-4 | 4°C |
| SA301834 | neg-10 | 4°C |
| SA301835 | neg2 | 4°C |
| SA301836 | neg6 | 4°C |
| SA301837 | posi36 | 4°C |
| SA301838 | neg-14 | 4°C |
| SA301839 | posi38 | 4°C |
| SA301840 | neg-12 | 4°C |
| SA301841 | gWAT8 | 4°C |
| SA301842 | neg-34 | 4°C |
| SA301843 | posi-10 | 4°C |
| SA301844 | posi18 | 4°C |
| SA301845 | gWAT4 | 4°C |
| SA301846 | posi-8 | 4°C |
| SA301847 | neg-36 | 4°C |
| SA301848 | posi20 | 4°C |
| SA301849 | posi-12 | 4°C |
| SA301850 | posi-14 | 4°C |
| SA301851 | posi-20 | 4°C |
