Summary of Study ST002813

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001760. The data can be accessed directly via it's Project DOI: 10.21228/M86T5X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002813
Study Title	Effects of acute cold exposure on mouse metabolome
Study Summary	Cold-induced thermogenesis is widely studied as a potential avenue to treat obesity, but a thorough understanding of the metabolic changes driving thermogenesis is lacking. We analyzed mouse plasma and tissue samples from fasted mice housed at room temperature or acutely exposed to 4°C. First, C57BL/6 mice (n=41) were divided into 2 groups (n=20-21 per group) and underwent surgery to implant an indwelling arterial catheter. Mice originally housed at room temperature were housed without food for 6 hours at room temperature or 4°C, after which blood was collected from the arterial catheter and centrifuged, and plasma was collected for metabolomic analyses. Second, C57BL/6 mice (n=12) were divided into 2 groups (n=6 per group) and housed without food for 6 hours at room temperature or 4°C. Mice were euthanized, and tissues (brown adipose tissue, heart, liver, quadricepts muscle, diaphragm, and gonadal-white adipose tissue) were collected, freeze-clamped in liquid nitrogen, and used for metabolomic analyses. Overall our data detail a coordinated and broad metabolic response governing the thermogenic response to acute cold exposure in mice.
Institute	University of Pennsylvania
Department	Medicine
Laboratory	Arany Lab
Last Name	Bornstein
First Name	Marc
Address	3400 Civic Center Blvd (Smilow 11-187)
Email	marc.bornstein@pennmedicine.upenn.edu
Phone	7742796684
Submit Date	2023-08-09
Num Groups	4
Total Subjects	53
Num Males	53
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2023-10-05
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002927
Sampleprep Summary:	For extraction of water-soluble metabolites from plasma samples, 200 µl of methanol was added to 5 µl plasma. This mixture was incubated on ice for 10 minutes, then centrifuged at 17,000g for 10 minutes at 4°C. The supernatant was transferred to an MS vial to run on LCMS. For extraction of hydrophobic metabolites (lipids) from plasma samples, 120 µl of isopropanol kept on dry ice was added to 3 µl plasma. This mixture was vortexed for 10 seconds and kept on dry ice for 10 minutes, then centrifuged at 17,000g for 10 minutes at 4°C. The supernatant was loaded into glass inserts in an MS vial to run on LCMS. For extraction of water-soluble metabolites from tissue samples, frozen tissues were first ground to a powder using a cryomill (Restch, Newtown, PA). Extraction solution kept on ice (40:40:20 methanol:acetonitrile:water) was added to ~10 mg of ground tissue (40 µl solution per mg tissue), vortexed, and kept on ice for 10 minutes, then centrifuged at 17,000g for 10 minutes at 4°C. The supernatant was collected and centrifuged again at the same speed for 10 minutes, and this second supernatant was transferred to an MS vial to run on LCMS.

Sampleprep ID:

SP002927

Sampleprep Summary:

For extraction of water-soluble metabolites from plasma samples, 200 µl of methanol was added to 5 µl plasma. This mixture was incubated on ice for 10 minutes, then centrifuged at 17,000g for 10 minutes at 4°C. The supernatant was transferred to an MS vial to run on LCMS. For extraction of hydrophobic metabolites (lipids) from plasma samples, 120 µl of isopropanol kept on dry ice was added to 3 µl plasma. This mixture was vortexed for 10 seconds and kept on dry ice for 10 minutes, then centrifuged at 17,000g for 10 minutes at 4°C. The supernatant was loaded into glass inserts in an MS vial to run on LCMS. For extraction of water-soluble metabolites from tissue samples, frozen tissues were first ground to a powder using a cryomill (Restch, Newtown, PA). Extraction solution kept on ice (40:40:20 methanol:acetonitrile:water) was added to ~10 mg of ground tissue (40 µl solution per mg tissue), vortexed, and kept on ice for 10 minutes, then centrifuged at 17,000g for 10 minutes at 4°C. The supernatant was collected and centrifuged again at the same speed for 10 minutes, and this second supernatant was transferred to an MS vial to run on LCMS.