Summary of Study ST003076
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001916. The data can be accessed directly via it's Project DOI: 10.21228/M82B0Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003076 |
| Study Title | Data-dependent and -independent acquisition lipidomics analysis reveals the tissue-dependent effect of metformin on lipid metabolism |
| Study Summary | Free fatty acids and polar metabolite analysis were conducted on six mouse tissues and plasma using HILIC chromatography coupled to a Q Exactive Plus mass spectrometer. This enabled a comprehensive assessment of fatty acid and metabolic changes induced by metformin across various tissues. |
| Institute | North Carolina State University |
| Last Name | Liu |
| First Name | Xiaojing |
| Address | 128 Polk Hall, 120 W Broughton Dr. |
| xliu68@ncsu.edu | |
| Phone | 919.515.4387 |
| Submit Date | 2024-02-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005034 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Xbridge Amide (100 × 2.1 mm, 3.5 um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | arbitrary unit |
Chromatography:
| Chromatography ID: | CH003805 |
| Chromatography Summary: | A hydrophilic interaction chromatography method (HILIC) with an Xbridge amide column (100 x 2.1 mm i.d., 3.5 m; Waters) was used for compound separation at 25 °C. Mobile phase A: water with 5 mM ammonium acetate (pH 6.8), and mobile phase B: 100% acetonitrile. Linear gradient is: 0 min, 85% B; 1.5 min, 85% B; 5.5 min, 35% B; 6.9 min, 35% B; 10.5 min, 35% B; 10.6 min, 10% B; 12.5 min, 10% B; 13.5 min, 85% B; 17.9 min, 85% B; 18 min, 85% B; 20 min, 85% B. For Ultimate 3000 UHPLC, the flow rate is: 0-5.5 min, 0.15 ml/min; 6.9-10.5 min, 0.17 ml/min; 10.6-17.9 min, 0.3 ml/min; 18-20 min, 0.15 ml/min. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Xbridge Amide (100 × 2.1 mm, 3.5 um) |
| Column Temperature: | 25 °C |
| Flow Gradient: | Linear gradient is: 0 min, 85% B; 1.5 min, 85% B; 5.5 min, 35% B; 6.9 min, 35% B; 10.5 min, 35% B; 10.6 min, 10% B; 12.5 min, 10% B; 13.5 min, 85% B; 17.9 min, 85% B; 18 min, 85% B; 20 min, 85% B. |
| Flow Rate: | 0.15 ml/min |
| Solvent A: | 100% water; 5 mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
