Summary of Study ST003076

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001916. The data can be accessed directly via it's Project DOI: 10.21228/M82B0Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003076
Study Title	Data-dependent and -independent acquisition lipidomics analysis reveals the tissue-dependent effect of metformin on lipid metabolism
Study Summary	Free fatty acids and polar metabolite analysis were conducted on six mouse tissues and plasma using HILIC chromatography coupled to a Q Exactive Plus mass spectrometer. This enabled a comprehensive assessment of fatty acid and metabolic changes induced by metformin across various tissues.
Institute	North Carolina State University
Last Name	Liu
First Name	Xiaojing
Address	128 Polk Hall, 120 W Broughton Dr.
Email	xliu68@ncsu.edu
Phone	919.515.4387
Submit Date	2024-02-07
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-03-01
Release Version	1

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP003197
Sampleprep Summary:	All tissue sample was first homogenized in liquid nitrogen and then 10-20 mg tissues were weighed into a new 1.7 ml Eppendorf tube. Ice cold extraction solvent (400 l 80% methanol/water) and 10 l metformin-d6 (50 ng/l in water) was added to each sample. Geno/Grinder homogenizer was used (1500 rpm, 1 to 2 min) to further break down the tissue chunk and form an even suspension. 200 ul supernatant containing polar metabolites was removed after centrifugation at 20,000 g at 4 °C for 10 min and transferred to LC vial for polar metabolite analysis using LC-HRMS. To extract metabolites and lipids from mouse plasma, 10 l plasma was mixed with 10 l water containing internal standards (50 ng/l metformin-d6, 5 mM [U-13C]-glucose and [U-13C]-lactate, and 80 l ice cold methanol was added. After vortex for 1 min, the mixture was centrifuged with a speed of 20,000 g at 4 °C for 10 min, and 20 l was transferred directly to LC vial without solvent evaporation, followed by LC-HRMS analysis (injection volume, 3 l) of polar metabolites such as glucose and metformin.

Sampleprep ID:

SP003197

Sampleprep Summary:

All tissue sample was first homogenized in liquid nitrogen and then 10-20 mg tissues were weighed into a new 1.7 ml Eppendorf tube. Ice cold extraction solvent (400 l 80% methanol/water) and 10 l metformin-d6 (50 ng/l in water) was added to each sample. Geno/Grinder homogenizer was used (1500 rpm, 1 to 2 min) to further break down the tissue chunk and form an even suspension. 200 ul supernatant containing polar metabolites was removed after centrifugation at 20,000 g at 4 °C for 10 min and transferred to LC vial for polar metabolite analysis using LC-HRMS. To extract metabolites and lipids from mouse plasma, 10 l plasma was mixed with 10 l water containing internal standards (50 ng/l metformin-d6, 5 mM [U-13C]-glucose and [U-13C]-lactate, and 80 l ice cold methanol was added. After vortex for 1 min, the mixture was centrifuged with a speed of 20,000 g at 4 °C for 10 min, and 20 l was transferred directly to LC vial without solvent evaporation, followed by LC-HRMS analysis (injection volume, 3 l) of polar metabolites such as glucose and metformin.