Summary of Study ST003076
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001916. The data can be accessed directly via it's Project DOI: 10.21228/M82B0Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003076 |
| Study Title | Data-dependent and -independent acquisition lipidomics analysis reveals the tissue-dependent effect of metformin on lipid metabolism |
| Study Summary | Free fatty acids and polar metabolite analysis were conducted on six mouse tissues and plasma using HILIC chromatography coupled to a Q Exactive Plus mass spectrometer. This enabled a comprehensive assessment of fatty acid and metabolic changes induced by metformin across various tissues. |
| Institute | North Carolina State University |
| Last Name | Liu |
| First Name | Xiaojing |
| Address | 128 Polk Hall, 120 W Broughton Dr. |
| xliu68@ncsu.edu | |
| Phone | 919.515.4387 |
| Submit Date | 2024-02-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005034 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Xbridge Amide (100 × 2.1 mm, 3.5 um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | arbitrary unit |
MS:
| MS ID: | MS004773 |
| Analysis ID: | AN005034 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | When Q Exactive Plus mass spectrometer was used, the relevant parameters are as listed: heater temperature, 120 °C; sheath gas, 30; auxiliary gas, 10; sweep gas, 3; spray voltage, 3.6 kV for positive mode and 2.5 kV for negative mode; capillary temperature, 320°C; S-lens, 55. The resolution was set at 70,000 (at m/z 200). Maximum injection time (max IT) was set at 200 ms and automatic gain control (AGC) was set at 3 × 106. FA species were detected using amide column and the identification was based on MS1 and the retention time determined by standard compounds when available. Integrated peak area of analytes was used to calculate the fold change of analytes in different samples. Metabolite and free fatty acid analysis was performed using Sieve (Thermo Fisher Scientific) based on theoretical m/z and retention time determined based on standard compounds. |
| Ion Mode: | UNSPECIFIED |
