Summary of study ST000244

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000197. The data can be accessed directly via it's Project DOI: 10.21228/M8XK51 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000244
Study TitleMetabolomic Diagnosis in Horse
Study Typecomparison
Study SummaryIn this project we will investigate the feasibility of metabolomics and to characterize the diversity of “metabotypes” in the horse, towards discovery of markers and pathways associated with obesity and insulin resistance in the equine model. The collaborative team of researchers assembled for this work have identified horses severely affected with Equine Metabolic syndrome, often characterized by obesity and hyperinsulinemia. These animals are all from the Arabian breed, to control for some genetic background. Horses are age, sex and farm of residence matched with a control animal whenever possible. Carefully controlled collection protocols were utilized to ensure minimal variability in sample age and quality. Blood plasma is submitted for both global LC-MS analysis through the SECIM core facilities. This discovery-based approach will begin to generate new targets for the development of novel therapeutic interventions for the treatment and prevention of obesity, type-2 diabetes as well as related secondary conditions in both humans and horses. Finally, as the first dataset of its kind in the horse, we may also be able to highlight promising new biomarkers for veterinary diagnostic use.
University of Florida
Last NameBrooks
First NameSamantha
Submit Date2015-10-13
Num Groups3
Total Subjects51
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Samantha Brooks Samantha Brooks application/zip

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Collection ID:CO000255
Collection Summary:A mobile set-up allowed for immediate processing of samples from study horses on their home farm. 50mL of blood was collected into two EDTA tubes, two no-additive tubes, and one sodium oxalate tube. EDTA and sodium oxalate treated samples were thoroughly mixed by inversion and immediately centrifuged to pellet the cells and allow for removal of plasma by pipette. Blood drawn in a no-additive tube was allowed to clot, centrifuged, and had the serum removed by pipette. The draw yielded 6-8 mL of plasma and 3-4mL of serum, which was then divided in to 1mL aliquots and immediately snap frozen by immersion in liquid nitrogen. Upon returning to the lab, the samples were then transfered to -80C for storage. Currently, only plasma is being submitted for metabolomic analysis.
Collection Protocol Filename:collection.txt
Sample Type:Blood
Collection Method:jugular venipuncture
Collection Location:On farm
Collection Duration:30 minutes per horse total
Collection Time:na
Volumeoramount Collected:50ml whole blood
Storage Conditions:snap frozen in LN2 on farm, -80 in lab
Collection Vials:glass vacutainer
Storage Vials:plastic cryovials
Collection Tube Temp:ambient during draw and centrifugation, then LN2 after plasma removal
Blood Serum Or Plasma:PLASMA