Summary of Study ST001298
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000879. The data can be accessed directly via it's Project DOI: 10.21228/M82M47 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001298 |
| Study Title | Metabolome Profiling of Synechococcus elongatus PCC 11801 strains engineered for Succinate Production |
| Study Type | Measurement of relative metabolite pools of wild type and engineered Synechococcus elongatus PCC 11801 strains using Isotopic Ratio Method |
| Study Summary | Experiments to measure relative metabolite pools of wild type Synechococcus elongatus PCC 11801 and its recombinants producing succinate. The wild type and the engineered strains producing succinate were cultivated at 1% CO2 and their metabolome data was collected in three biological and three technical replicates (n=9). The study aims to find metabolomics changes between the wild type and the engineered to identify potential rate-limiting steps that be used as targets for improved production. |
| Institute | Indian Institute of Technology Bombay |
| Department | Department of Chemical Engineering |
| Laboratory | Bio systems Engineering Lab |
| Last Name | Wangikar |
| First Name | Pramod P |
| Address | Department of Chemical Engineering, IIT Bombay, Powai, Mumbai, Maharashtra, India - 400076 |
| wangikar@iitb.ac.in | |
| Phone | 22254215 |
| Submit Date | 2020-01-03 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-07-05 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001367 |
| Collection Summary: | The experiment was conducted in a shaker maintained at a temperature of 38°C, 1% CO2, and 120 rpm. The cells were grown in 100 ml shake flasks with 20 ml culture volume and the samples for metabolomics analysis were collected at a metabolic steady-state (OD 730 nm = 0.6). Samples were quenched with methanol and extracted using the methanol-chloroform-water method. Extracts were stored at -80°C till LCMS analysis. LCMS analysis was done in the negative ion mode using information-dependent acquisition (IDA) method. |
| Sample Type: | Bacterial cells |
| Collection Tube Temp: | 4 degree centrigrade |
