Summary of Study ST001438
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000988. The data can be accessed directly via it's Project DOI: 10.21228/M80680 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001438 |
| Study Title | Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples - MSC |
| Study Summary | The human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. A second example to illustrate TENGi MS capabilities involves rare cell metabolomics of cultured mesenchymal stromal cells (MSCs), a cell type that has shown potential for treating a variety of chronic diseases. Examination of metabolic changes of MSCs cultured under conditions that may impact in vitro therapeutic activity, such as aggregate culture, or preconditioning with interferon gamma (IFN- γ)13, is critical for identifying attributes of cell quality. Reducing cell numbers required to perform MSC metabolomic analysis is essential for improving the manufacturing of highly therapeutic MSCs without significantly impeding production. |
| Institute | Georgia Institute of Technology |
| Last Name | Fernandez |
| First Name | Facundo |
| Address | 901 Atlantic Dr NE, Atlanta, GA, 30332, USA |
| fernandez@gatech.edu | |
| Phone | 404-385-4432 |
| Submit Date | 2020-08-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2020-09-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO001507 |
| Collection Summary: | Bone marrow-derived MSCs (RoosterBio Inc., Lot #000139) were expanded for two passages in culture after being received. They were frozen in ~5x105 aliquots in Cryostor CS10 freeze media (BioLife). Frozen aliquots were revived and plated in tissue culture polystyrene flasks (Corning) for 3-4 days prior to seeding onto test surfaces. MSCs were cultured in low-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals, lot E16063), and 1% antibiotic/antimycotic solution (Gibco). Once confluent, MSCs were washed with sterile-filtered phosphate-buffered saline (PBS, Thermo Fisher) and detached from flasks using TrypLE express (Thermo Fisher). Dissociated cells were counted using a hemacytometer and replated at 13,000 cells/cm2 in T-75 tissue culture flasks. After overnight incubation, MSCs then were exposed to 48 hours of culture media (control conditions), or culture media supplemented with 50 ng/mL IFN- γ (Thermo Fisher). MSCs were then washed with PBS and trypsinized as described above, and the number of harvested cells counted using a hemocytometer. |
| Sample Type: | Mesenchymal stromal cells |
