Summary of Study ST001438
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000988. The data can be accessed directly via it's Project DOI: 10.21228/M80680 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001438 |
| Study Title | Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples - MSC |
| Study Summary | The human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. A second example to illustrate TENGi MS capabilities involves rare cell metabolomics of cultured mesenchymal stromal cells (MSCs), a cell type that has shown potential for treating a variety of chronic diseases. Examination of metabolic changes of MSCs cultured under conditions that may impact in vitro therapeutic activity, such as aggregate culture, or preconditioning with interferon gamma (IFN- γ)13, is critical for identifying attributes of cell quality. Reducing cell numbers required to perform MSC metabolomic analysis is essential for improving the manufacturing of highly therapeutic MSCs without significantly impeding production. |
| Institute | Georgia Institute of Technology |
| Last Name | Fernandez |
| First Name | Facundo |
| Address | 901 Atlantic Dr NE, Atlanta, GA, 30332, USA |
| fernandez@gatech.edu | |
| Phone | 404-385-4432 |
| Submit Date | 2020-08-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2020-09-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000988 |
| Project DOI: | doi: 10.21228/M80680 |
| Project Title: | Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples |
| Project Summary: | The human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. Samples tested for this approach included exhaled breath condensates (EBC) collected from cystic fibrosis (CF) patients as well as in vitro-cultured human mesenchymal stromal cells (MSCs). Both test samples were only available in minimum amounts. Experiments showed that picoliter-volume spray pulses sufficed to generate high-quality spectral fingerprints, which increased the information density produced per unit sample volume. This TENGi nanoESI strategy has the potential to fill in the gap in metabolomics where liquid chromatography-MS-based analyses cannot be applied. Our method could open up new avenues for future investigations into understanding metabolic changes caused by diseases or external stimuli. |
| Institute: | Georgia Institute of Technology |
| Last Name: | Fernandez |
| First Name: | Facundo |
| Address: | 901 Atlantic Dr NE, Atlanta, GA, 30332, USA |
| Email: | fernandez@gatech.edu |
| Phone: | 404-385-4432 |
