Summary of Study ST001638
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001035. The data can be accessed directly via it's Project DOI: 10.21228/M8X39Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001638 |
Study Title | Metabolomics analysis of Vehicle (DMSO/PBS; (1:1) v/v) and DRB18 (10 mg/kg) treated A549 xenograft tumors |
Study Type | Anticancer compound treatment experiment in vivo |
Study Summary | A549 xenograft tumors were treated with (DMSO/PBS; (1:1) v/v) and DRB18 (10 mg/kg) for 5 weeks. The mice were then sacrificed and tumors were then excised. Tumors were then subjected to untargeted metabolomics analysis. |
Institute | Ohio University |
Department | Biological Sciences |
Laboratory | Dr. Xiaozhuo Chen, Edison biotechnology Institute |
Last Name | Shriwas |
First Name | Pratik |
Address | 172 Water Tower, Building 25, The Ridges, Konnekar Research Centerm Athens Ohio - 45701, USA |
ps774614@ohio.edu | |
Phone | 7406033801 |
Submit Date | 2020-12-07 |
Num Groups | 2 |
Total Subjects | 20 |
Num Males | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2021-04-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
Collection ID: | CO001708 |
Collection Summary: | A549 lung cancer xenograft tumors were obtained after sacrificing the mice. The tumors were then prepared according to the protocol described and metabolomics analysis was performed. |
Sample Type: | Tumor cells |
Collection Method: | Tumors were homogenized in a Beadbud homogenizer (Benchmark scientific) in a mixture of LC-MS grade ice-cold methanol and water (1:1; v/v). The supernatant was collected and sonicated in a water bath incubator for 15 minutes, followed by centrifugation at 13,000 rpm for 10 minutes and collection of the supernatant. |
Collection Location: | Homogenization vials |
Collection Frequency: | Once |
Collection Duration: | 4-5 minutes |
Volumeoramount Collected: | 1ml |
Storage Conditions: | -80℃ |
Collection Vials: | Polypropylene 1.5 ml tubes |
Storage Vials: | Polypropylene 1.5 ml |