Summary of Study ST001638

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001035. The data can be accessed directly via it's Project DOI: 10.21228/M8X39Q This work is supported by NIH grant, U2C- DK119886.


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Study IDST001638
Study TitleMetabolomics analysis of Vehicle (DMSO/PBS; (1:1) v/v) and DRB18 (10 mg/kg) treated A549 xenograft tumors
Study TypeAnticancer compound treatment experiment in vivo
Study SummaryA549 xenograft tumors were treated with (DMSO/PBS; (1:1) v/v) and DRB18 (10 mg/kg) for 5 weeks. The mice were then sacrificed and tumors were then excised. Tumors were then subjected to untargeted metabolomics analysis.
Ohio University
DepartmentBiological Sciences
LaboratoryDr. Xiaozhuo Chen, Edison biotechnology Institute
Last NameShriwas
First NamePratik
Address172 Water Tower, Building 25, The Ridges, Konnekar Research Centerm Athens Ohio - 45701, USA
Submit Date2020-12-07
Num Groups2
Total Subjects20
Num Males20
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2021-04-01
Release Version1
Pratik Shriwas Pratik Shriwas application/zip

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Sample Preparation:

Sampleprep ID:SP001721
Sampleprep Summary:Tumors were homogenized in a Beadbud homogenizer (Benchmark scientific) in a mixture of LC-MS grade ice-cold methanol and water (1:1; v/v). The supernatant was collected and sonicated in a water bath incubator for 15 minutes, followed by centrifugation at 13,000 rpm for 10 minutes and collection of the supernatant. Supernatants collected from in vitro and in vivo extraction were then lyophilized using a speed vacuum evaporator. The samples were then dissolved into a mixture of LC-MS grade acetonitrile/water (1:1; v/v) for analysis.
Sampleprep Protocol Filename:prats1988_20201207_Invivo_collection_and_preparation_method.docx
Processing Method:Metabolite extraction; Quenching; speed vacuum evaporation
Processing Storage Conditions:-80℃
Extraction Method:Quenching with ice cold methanol
Extract Enrichment:Speed vaccum evaporator
Sample Resuspension:Acetonitrile/waster (1:1)