Summary of Study ST001983
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001259. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX2N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001983 |
Study Title | Metabolomic Fingerprinting of Human High Grade Serous Ovarian Carcinoma Cell Lines |
Study Summary | Focusing on defining the metabolomic basis of intratumoral heterogeneity in ovarian cancer, the metabolic diversity of a panel of high grade serous ovarian carcinoma (HGSOC) cell-lines we investigated using a metabolomics platform that interrogate 731 compounds. Metabolic fingerprinting followed by 2-dimensional and 3-dimensional principal component analysis defined the heterogeneity of the HGSOC cells and clustered them into five distinct metabolic groups. An overall increase in the metabolites associated with aerobic glycolysis and phospholipid metabolism were observed in the majority of the cancer cells. A preponderant increase in the levels of metabolites involved in trans-sulfuration and glutathione synthesis was also observed. Subsets of HGSOC cells showed an increase in the levels of 5-Hydroxytryptamine, gamma-aminobutyric acid, or glutamate, pointing to their potential role as oncometabolites. In summary, our results identify increased glycolysis, phospholipid metabolism and amino acid metabolism with the resultant increase in the levels of 5-Hydoxytryptamine, GABA, and Glutamate as metabolomic correlates underlying the heterogeneity of ovarian cancer cell lines. |
Institute | University of Oklahoma Health Sciences Center |
Department | Cell Biology |
Laboratory | Danny N Dhanasekaran |
Last Name | Jayaraman |
First Name | Muralidharan |
Address | 975 NE 10th street, BRC1470, Oklahoma City, Oklahoma, 73104, USA |
Muralidharan-Jayaraman@ouhsc.edu | |
Phone | 4052718001 x 30492 |
Submit Date | 2021-09-20 |
Num Groups | 2 |
Total Subjects | 30 |
Num Females | 15 |
Study Comments | Ovarian cancer cell lines |
Analysis Type Detail | LC-MS |
Release Date | 2021-12-01 |
Release Version | 1 |
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Collection:
Collection ID: | CO002057 |
Collection Summary: | Immortalized normal fallopian-tube-derived epithelial cells (FTE188) have been previously described and used here (1). High grade serous carcinoma cell line OVCAR3, OVCAR8, OVKATE, SNU119, SNU251, OVCAR4, OVSAHO, and Kuramochi cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (Cellgro, Manassas, VA), COV362, COV318, OAW28 and CAOV3 cells were maintained in Dulbecco’s modified Eagle’s (DMEM) Medium (Cellgro, Manassas, VA). OV90 cells were maintained in MCDB105: M199 (1:1) Medium (Thermo Fisher Scientific, Waltham, MA) and TYKNU cells were maintained in Minimum Essential Medium (MEM) (Cellgro, Manassas, VA). ES-2 cells were maintained in McCoy’s 5A medium (Sigma-Aldrich, St. Louis, MO). All cells were maintained at 37°C in a 5% CO2 incubator. All media were supplemented with 10% FBS (Gemini Bio-Products, West Sacramento, CA), 50 U/mL penicillin, 50 μg/ml streptomycin (Cellgro, Manassas, VA). Cells were grown to 20 million cells and washed with cold PBS. Cells were collected by scraping them off the plates. |
Sample Type: | Ovarian cancer cells |
Storage Conditions: | -80℃ |