Summary of Study ST002024
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001286. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002024 |
| Study Title | Plasmodium falciparum stable-isotope carbon labeling to explore metabolic consequences of keto–acid dehydrogenase disruption |
| Study Summary | Plasmodium falciparum cells in culture were treated with respective universally labelled carbon-13 metabolites for 2.5 hours at standard culture concentrations (glucose or glutamine) or 5 mM (acetate). Metabolites were isolated using 90% methanol, dried, reconstituted in HPLC-grade water, and analyzed by HPLC/MS. Resulting data were analyzed and compiled to generate study data. |
| Institute | Pennsylvania State University |
| Department | Chemistry |
| Laboratory | Llinás Laboratory |
| Last Name | Llinás |
| First Name | Manuel |
| Address | W126 Millennium Science Complex, University Park, PENNSYLVANIA, 16802, USA |
| mul27@psu.edu | |
| Phone | 814-867-3444 |
| Submit Date | 2021-07-15 |
| Num Groups | 16 |
| Total Subjects | 146 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-10-03 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002099 |
| Collection Summary: | Plasmodium falciparum NF54 attB parasites containing different genetic backgrounds were cultured in RPMI 1640 medium and magnetically enriched to increase the infected to uninfected RBC ratio. Following hemocytometer counts, 1x10^8 parasites were measured per condition into 5 mL of total media for 2.5 hours in the presence of the universally labelled carbon-13 metabolite (glucose, glutamine, or acetate). Medium was aspirated until 1 mL remained on each sample, the sample was transferred to a micro-centrifuge tube, spun, and the medium was aspirated. |
| Collection Protocol Filename: | Metabolite_Extraction_for_LCMS_2017.pdf |
| Sample Type: | Cultured cells |
| Collection Location: | Millenium Science Complex, University Park, Pennsylvania |
| Storage Conditions: | -80℃ |
| Collection Vials: | 1.5 mL eppendorf |
| Storage Vials: | 1.5 mL eppendorf |
| Collection Tube Temp: | On ice |
| Tissue Cell Quantity Taken: | 1x10^8 cells per sample in 1 mL total volume |
