Summary of Study ST002024
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001286. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002024 |
| Study Title | Plasmodium falciparum stable-isotope carbon labeling to explore metabolic consequences of keto–acid dehydrogenase disruption |
| Study Summary | Plasmodium falciparum cells in culture were treated with respective universally labelled carbon-13 metabolites for 2.5 hours at standard culture concentrations (glucose or glutamine) or 5 mM (acetate). Metabolites were isolated using 90% methanol, dried, reconstituted in HPLC-grade water, and analyzed by HPLC/MS. Resulting data were analyzed and compiled to generate study data. |
| Institute | Pennsylvania State University |
| Department | Chemistry |
| Laboratory | Llinás Laboratory |
| Last Name | Llinás |
| First Name | Manuel |
| Address | W126 Millennium Science Complex, University Park, PENNSYLVANIA, 16802, USA |
| mul27@psu.edu | |
| Phone | 814-867-3444 |
| Submit Date | 2021-07-15 |
| Num Groups | 16 |
| Total Subjects | 146 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-10-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002112 |
| Sampleprep Summary: | Once samples were obtained, metabolism was quenched with 90% methanol containing 0.25 uM labeled aspartate, cells were centrifuged, and the supernatant was removed by nitrogen drying. Sample was reconstituted in 3% HPLC-grade methanol and run on the instrument. |
| Processing Method: | Wash, spin, quench, spin, dry, store at -80 C until run on the instrument, resuspend |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 90% methanol extraction |
| Extract Cleanup: | Centrifugation and nitrogen drying |
| Sample Resuspension: | 100 uL 3% methanol with 1 uM chlorpropamide |
| Sample Spiking: | 0.25 uM Labelled Aspartate, 1 uM chlorpropamide |
