Summary of Study ST002510
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001620. The data can be accessed directly via it's Project DOI: 10.21228/M89B04 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002510 |
| Study Title | Strain supernatants: Strain diversity of Eggerthella lenta metabolites in defined media |
| Study Type | Untargeted LC-MS |
| Study Summary | This dataset contains untargeted metabolomics analysis of supernatants from stationary phase of a collection of 30 strains of Eggerthella lenta grown in defined EDM1 media. |
| Institute | University of California, San Francisco |
| Last Name | Noecker |
| First Name | Cecilia |
| Address | 513 Parnassus Ave HSW1501, San Francisco, CA 94143 |
| cecilia.noecker@ucsf.edu | |
| Phone | 415-502-3264 |
| Submit Date | 2023-03-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-04-04 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002603 |
| Collection Summary: | For the comparative strain metabolomics experiment, 96-well polypropylene deep well plates were prepared with 800μL of fresh media in each well. Starter cultures and inocula for 29 isolates of Eggerthella lenta and 1 isolate of Eggerthella sinensis [26] were prepared as described above for growth assays, except without final dilution, and 80 μL was used to inoculate wells, leaving a blank well in between every culture well to prevent cross-contamination. After 72 hours, OD600 measurements were taken, plates were centrifuged, and supernatants were collected. Plates were centrifuged at 1,928 rcf at 4°C for 8 minutes, after which supernatants were collected into fresh polypropylene tubes or plates, sealed, and flash-frozen in liquid nitrogen. |
| Sample Type: | Bacterial culture supernatant |
| Collection Frequency: | single time point (72 hours) |
| Storage Conditions: | -80℃ |
