Summary of Study ST001950

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001237. The data can be accessed directly via it's Project DOI: 10.21228/M8TB0S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001950
Study TitleLipidome Alterations Following Mild Traumatic Brain Injury.
Study TypeUntargeted Lipidomics
Study SummaryTraumatic brain injury (TBI) poses a major health challenge, with tens of millions of new cases reported globally every year. Brain damage resulting from TBI can vary significantly due to factors including injury severity, diffusivity, modality, time delay relative to impact, and exposure to repeated injury events. Untargeted lipidomic analysis of Sprague-Dawley rat serum within 24 hours of mild single and repeat controlled cortical impact (CCI) injury events led to the discovery of biomarker candidates of TBI. Lipid biomarkers have a unique potential to serve as objective molecular measures of the body’s response to injury as their alteration in brain tissue can be more freely observed than for larger protein markers. Animal serum was analyzed via ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) in positive and negative ion modes. Known lipid species were identified through matching to in-house tandem MS databases. Machine learning and feature selection approaches were used to construct lipid panels capable of distinguishing serum from injured and uninjured animals across a range of injury severities and timepoints within the first day of injury. The best multivariate lipid panels had over 90% cross-validated sensitivity, selectivity, and accuracy and consisted of species from nine different lipid classes. These mapped onto sphingolipid signaling, autophagy, necroptosis and glycerophospholipid metabolism pathways, with FDR corrected p-values better than 0.05.
Institute
Georgia Institute of Technology
DepartmentChemistry and Biochemistry
LaboratoryFacundo Fernández
Last NameGier
First NameEric
Address311 Ferst Drive, Atlanta, GA, 30318, USA
Emailegier3@gatech.edu
Phone2246221699
Submit Date2021-10-24
Num Groups6
Total Subjects32
Num Males14
Num Females18
Study CommentsLC-MS
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-02-07
Release Version1
Eric Gier Eric Gier
https://dx.doi.org/10.21228/M8TB0S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Injury Severity Sex Blood Collection
SA18392428D_T4_I3_G1_L1Repeat-Impact F 24 hour
SA18392520D_T4_I3_G1_L1Repeat-Impact F 24 hour
SA18392630D_T4_I3_G1_L1Repeat-Impact F 24 hour
SA18392732D_T4_I3_G1_L1Repeat-Impact F 24 hour
SA18392823D_T4_I3_G1_L1Repeat-Impact F 24 hour
SA18392924B_T2_I3_G1_L1Repeat-Impact F 30 min
SA18393023B_T2_I3_G1_L1Repeat-Impact F 30 min
SA18393116B_T2_I3_G1_L1Repeat-Impact F 30 min
SA18393228B_T2_I3_G1_L1Repeat-Impact F 30 min
SA18393332B_T2_I3_G1_L1Repeat-Impact F 30 min
SA18393420B_T2_I3_G1_L1Repeat-Impact F 30 min
SA18393520C_T3_I3_G1_L1Repeat-Impact F 4 hour
SA18393630B_T2_I3_G1_L1Repeat-Impact F 4 hour
SA18393728C_T3_I3_G1_L1Repeat-Impact F 4 hour
SA18393816C_T3_I3_G1_L1Repeat-Impact F 4 hour
SA18393930C_T3_I3_G1_L1Repeat-Impact F 4 hour
SA18394023C_T3_I3_G1_L2Repeat-Impact F 4 hour
SA18394132C_T3_I3_G1_L1Repeat-Impact F 4 hour
SA18394224C_T3_I3_G1_L1Repeat-Impact F 4 hour
SA18394330A_T1_I3_G1_L1Repeat-Impact F Baseline
SA18394432A_T1_I3_G1_L1Repeat-Impact F Baseline
SA18394523A_T1_I3_G1_L1Repeat-Impact F Baseline
SA18394620A_T1_I3_G1_L1Repeat-Impact F Baseline
SA18394716A_T1_I3_G1_L1Repeat-Impact F Baseline
SA18394824A_T1_I3_G1_L1Repeat-Impact F Baseline
SA18394928A_T1_I3_G1_L1Repeat-Impact F Baseline
SA18395011D_T4_I3_G2_L1Repeat-Impact M 24 hour
SA1839519D_T4_I3_G2_L1Repeat-Impact M 24 hour
SA18395212D_T4_I3_G2_L1Repeat-Impact M 24 hour
SA1839534D_T4_I3_G2_L1Repeat-Impact M 24 hour
SA18395411B_T2_I3_G2_L1Repeat-Impact M 30 min
SA18395512B_T2_I3_G2_L1Repeat-Impact M 30 min
SA18395612C_T3_I3_G2_L1Repeat-Impact M 4 hour
SA18395711C_T3_I3_G2_L1Repeat-Impact M 4 hour
SA1839584C_T3_I3_G2_L1Repeat-Impact M 4 hour
SA1839599C_T3_I3_G2_L1Repeat-Impact M 4 hour
SA1839609A_T1_I3_G2_L1Repeat-Impact M Baseline
SA18396111A_T1_I3_G2_L1Repeat-Impact M Baseline
SA18396212A_T1_I3_G2_L1Repeat-Impact M Baseline
SA1839634A_T1_I3_G2_L1Repeat-Impact M Baseline
SA18396418D_T4_I1_G1_L1Sham F 24 hour
SA18396521D_T4_I1_G1_L1Sham F 24 hour
SA18396631D_T4_I1_G1_L1Sham F 24 hour
SA18396719D_T4_I1_G1_L1Sham F 24 hour
SA18396814D_T4_I1_G1_L1Sham F 24 hour
SA18396931B_T2_I1_G1_L1Sham F 30 min
SA18397018B_T2_I1_G1_L1Sham F 30 min
SA18397119B_T2_I1_G1_L2Sham F 30 min
SA18397214B_T2_I1_G1_L1Sham F 30 min
SA18397331C_T3_I1_G1_L1Sham F 4 hour
SA18397421C_T3_I1_G1_L2Sham F 4 hour
SA18397518C_T3_I1_G1_L1Sham F 4 hour
SA18397614C_T3_I1_G1_L1Sham F 4 hour
SA18397719C_T3_I1_G1_L1Sham F 4 hour
SA18397818A_T1_I1_G1_L1Sham F Baseline
SA18397914A_T1_I1_G1_L1Sham F Baseline
SA18398031A_T1_I1_G1_L1Sham F Baseline
SA18398125D_T4_I1_G2_L1Sham M 24 hour
SA1839822D_T4_I1_G2_L1Sham M 24 hour
SA1839831D_T4_I1_G2_L1Sham M 24 hour
SA1839847D_T4_I1_G2_L1Sham M 24 hour
SA1839855D_T4_I1_G2_L1Sham M 24 hour
SA18398626D_T4_I1_G2_L1Sham M 24 hour
SA1839877B_T2_I1_G2_L1Sham M 30 min
SA18398825B_T2_I1_G2_L1Sham M 30 min
SA18398926B_T2_I1_G2_L1Sham M 30 min
SA1839905B_T2_I1_G2_L1Sham M 30 min
SA1839912B_T2_I1_G2_L1Sham M 30 min
SA1839921C_T3_I1_G2_L1Sham M 4 hour
SA1839932C_T3_I1_G2_L1Sham M 4 hour
SA1839945C_T3_I1_G2_L1Sham M 4 hour
SA1839957C_T3_I1_G2_L1Sham M 4 hour
SA18399625C_T3_I1_G2_L1Sham M 4 hour
SA18399726C_T3_I1_G2_L1Sham M 4 hour
SA18399826A_T1_I1_G2_L1Sham M Baseline
SA1839995A_T1_I1_G2_L1Sham M Baseline
SA1840002A_T1_I1_G2_L1Sham M Baseline
SA18400125A_T1_I1_G2_L1Sham M Baseline
SA1840027A_T1_I1_G2_L1Sham M Baseline
SA18400327D_T4_I2_G1_L1Single-Impact F 24 hour
SA18400422D_T4_I2_G1_L1Single-Impact F 24 hour
SA18400517D_T4_I2_G1_L1Single-Impact F 24 hour
SA18400629D_T4_I2_G1_L1Single-Impact F 24 hour
SA18400717B_T2_I2_G1_L1Single-Impact F 30 min
SA18400815B_T2_I2_G1_L1Single-Impact F 30 min
SA18400929B_T2_I2_G1_L1Single-Impact F 30 min
SA18401027B_T2_I2_G1_L1Single-Impact F 30 min
SA18401113B_T2_I2_G1_L2Single-Impact F 30 min
SA18401227C_T3_I2_G1_L1Single-Impact F 4 hour
SA18401329C_T3_I2_G1_L1Single-Impact F 4 hour
SA18401417C_T3_I2_G1_L1Single-Impact F 4 hour
SA18401515C_T3_I2_G1_L1Single-Impact F 4 hour
SA18401622C_T3_I2_G1_L1Single-Impact F 4 hour
SA18401713C_T3_I2_G1_L1Single-Impact F 4 hour
SA18401813A_T1_I2_G1_L1Single-Impact F Baseline
SA18401929A_T1_I2_G1_L1Single-Impact F Baseline
SA18402015A_T1_I2_G1_L1Single-Impact F Baseline
SA18402127A_T1_I2_G1_L1Single-Impact F Baseline
SA18402222A_T1_I2_G1_L1Single-Impact F Baseline
SA18402317A_T1_I2_G1_L1Single-Impact F Baseline
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