Summary of Study ST001073
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000718. The data can be accessed directly via it's Project DOI: 10.21228/M8VT2P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001073 |
| Study Title | Lipid profiling of Wnt3a-induced optic nerve regeneration |
| Study Type | untargeted lipid profiling |
| Study Summary | We analyzed lipid profiles of mouse retina and optic nerve for 2 time points - 7 and 15 days post-crush, and 3 conditions - intact control, optic nerve crush + vehicle (saline) intravitreal injection, optic nerve crush + 20 ng of Wnt3a injection. |
| Institute | University of Miami |
| Department | Ophthalmology, Bascom Palmer Eye Institute |
| Laboratory | Sanjoy K. Bhattacharya lab |
| Last Name | Bhattacharya |
| First Name | Sanjoy |
| Address | Bascom Palmer Eye Institute (McKnight Bldg.), 1638 NW 10th Avenue, Suite 707A, University of Miami, Miami, FL, 33136 |
| sbhattacharya@med.miami.edu | |
| Phone | 305-482-4103 |
| Submit Date | 2018-10-03 |
| Num Groups | 6 |
| Total Subjects | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-12-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001755 |
|---|---|
| Chromatography ID | CH001239 |
| MS ID | MS001623 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Accela 600 |
| Column | Thermo Acclaim C30 (150 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | relative abundance (main area) |
MS:
| MS ID: | MS001623 |
| Analysis ID: | AN001755 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | positive and negative mode aligned together |
| Ion Mode: | UNSPECIFIED |
| Capillary Temperature: | 310˚C (negative mode) or 350˚C (positive mode) |
| Collision Energy: | 15, 30, 45, 60, 75, 90 (in positive and negative mode separately; total 12 runs per sample) |
| Ion Spray Voltage: | 4.4 kV |
