Summary of study ST001401

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000961. The data can be accessed directly via it's Project DOI: 10.21228/M8GD71 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001401
Study TitleSteady-state metabolomics time course of Saccharomyces cerevisiae mitochondrial fatty acid synthesis (mtFAS) mutants
Study TypeSteady-state targeted and untargeted metabolomics time course
Study SummaryThe goal of this work was to analyze metabolic changes in yeast at various time points with either the oar1 KO or the mct1 knock-out conditions when compared to a time-matched wild-type samples using gas chromatography-mass spectrometry (GC-MS).
Institute
University of Utah
DepartmentBiochemistry
LaboratoryRutter
Last NameBerg
First NameJordan
Address15 N Medical Drive East RM 5520, Salt Lake City, UT 84112-5650 USA
Emailjordan.berg@biochem.utah.edu
Phone(801) 581 3340
Submit Date2020-06-10
Num Groups3
Total Subjects95
Raw Data AvailableYes
Raw Data File Type(s).xml;csv;bin
Analysis Type DetailGC-MS
Release Date2020-06-22
Release Version1
Jordan Berg Jordan Berg
https://dx.doi.org/10.21228/M8GD71
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002343
Analysis type MS
Chromatography type GC
Chromatography system Agilent 5977b
Column Phenomenex Zebron AB-5HT (5m)
MS Type EI
MS instrument type HES-MSD
MS instrument name Agilent 5977
Ion Mode POSITIVE
Units AUC (unitless)

MS:

MS ID:MS002185
Analysis ID:AN002343
Instrument Name:Agilent 5977
Instrument Type:HES-MSD
MS Type:EI
MS Comments:All GC-MS analysis was performed with an Agilent 5977b GC-MS MSD-HES and an Agilent 7693A automatic liquid sampler. Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath. 25 µL of this solution was added to auto sampler vials. 60 µL of N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, Thermo #TS48913) was added automatically via the auto sampler and incubated for 30 minutes at 37 °C. After incubation, samples were vortexed and 1 µL of the prepared sample was injected into the gas chromatograph inlet in the split mode with the inlet temperature held at 250°C. A 10:1 split ratio was used for analysis of the majority of metabolites. For those metabolites that saturated the instrument at the 10:1 split concentration, a split of 50:1 was used for analysis. The gas chromatograph had an initial temperature of 60°C for one minute followed by a 10°C/min ramp to 325°C and a hold time of 5 minutes. A 30-meter Phenomenex Zebron AB-5HT with 5m inert Guardian capillary column was employed for chromatographic separation. Helium was used as the carrier gas at a rate of 1 mL/min. Data was collected using MassHunter software (Agilent). Metabolites were identified and their peak area was recorded using MassHunter Quant. This data was transferred to an Excel spread sheet (Microsoft, Redmond WA). Metabolite identity was established using a combination of an in-house metabolite library developed using pure purchased standards, the NIST library and the Fiehn library.
Ion Mode:POSITIVE
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