Summary of study ST001401

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000961. The data can be accessed directly via it's Project DOI: 10.21228/M8GD71 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001401
Study TitleSteady-state metabolomics time course of Saccharomyces cerevisiae mitochondrial fatty acid synthesis (mtFAS) mutants
Study TypeSteady-state targeted and untargeted metabolomics time course
Study SummaryThe goal of this work was to analyze metabolic changes in yeast at various time points with either the oar1 KO or the mct1 knock-out conditions when compared to a time-matched wild-type samples using gas chromatography-mass spectrometry (GC-MS).
University of Utah
Last NameBerg
First NameJordan
Address15 N Medical Drive East RM 5520, Salt Lake City, UT 84112-5650 USA
Phone(801) 581 3340
Submit Date2020-06-10
Num Groups3
Total Subjects95
Raw Data AvailableYes
Raw Data File Type(s).xml;csv;bin
Analysis Type DetailGC-MS
Release Date2020-06-22
Release Version1
Jordan Berg Jordan Berg application/zip

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Sample Preparation:

Sampleprep ID:SP001483
Sampleprep Summary:To each sample was added boiling 75% ethanol (EtOH) solution containing the internal standard d4-succinic acid (Sigma 293075). Boiling samples were vortexed and incubated at 90°C for 5 min. Samples were then incubated at -20 ˚C for 1 hr. After incubation the samples were centrifuged at 5,000 x g for 10 minutes at 4˚C. The supernatant was then transferred from each sample tube into a labeled, fresh 13x100mm glass culture tube. A second standard was then added (d27-myristic acid CDN Isotopes: D-1711). Pooled quality control samples were made by removing a fraction of collected supernatant from each sample and process blanks were made using only extraction solvent and no cell culture. The samples were then dried en vacuo. This process was completed in three separate batches.