Summary of Study ST001738

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001112. The data can be accessed directly via it's Project DOI: 10.21228/M8ZM49 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001738
Study TitleAdipoAtlas: A Reference Lipidome for Human White Adipose Tissue
Study SummaryObesity, characterized by expansion and metabolic dysregulation of white adipose tissue (WAT), has reached pandemic proportions and acts as a primer for a wide range of metabolic disorders. Remodelling of WAT lipidome in obesity and associated comorbidities can explain disease etiology and provide valuable diagnostic and prognostic markers. To support understanding of WAT lipidome remodelling at molecular level, we performed in-depth lipidomics profiling of human subcutaneous and visceral WAT of lean and obese individuals. Tissue-tailored preanalytical and analytical workflows allowed accurate identification and semi-absolute quantification of 1636 and 737 lipid molecular species, respectively, and summarized here in a form of human WAT reference lipidome. Deep lipidomic profiling allowed to identify main lipid (sub)classes undergoing depot/phenotype specific remodelling. Furthermore, previously unanticipated diversity of WAT ceramides was uncovered. AdipoAtlas reference lipidome will serve as a data-rich resource for development of WAT-specific high-throughput methods and as a scaffold for systems medicine data integration.
Institute
University of Leipzig
DepartmentFaculty for Chemistry and Mineralogy, Biotechnological-Biomedical Center
LaboratoryFedorova Lab
Last NameFedorova
First NameMaria
AddressDeutscher Platz 5
Emailmaria.fedorova@bbz.uni-leipzig.de
Phone03419731336
Submit Date2021-01-11
Num Groups4
Study CommentsPools of subcutaneous and visceral white adipose tissue were generated from lean patients (BMI < 25; n=5) and obese (BMI > 40; n=81)
Publicationshttps://doi.org/10.1016/j.xcrm.2021.100407
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-04-23
Release Version1
Maria Fedorova Maria Fedorova
https://dx.doi.org/10.21228/M8ZM49
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002829 AN002830 AN002831
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase HILIC
Chromatography system Vanquish Horizon Vanquish Horizon Vanquish Horizon
Column Thermo Accucore C30 (150 x 2.1mm,2.6um) Thermo Accucore C18 (150 x 2.1mm,2.6um) Waters ACQUITY UPLC BEH HILIC (100 x 1.0mm,1.7um,130 Å)
MS Type ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap; Thermo Fusion Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode UNSPECIFIED UNSPECIFIED POSITIVE
Units fmol/μg protein fmol/μg protein fmol/μg protein

MS:

MS ID:MS002622
Analysis ID:AN002829
Instrument Name:Thermo Q Exactive Plus Orbitrap; Thermo Fusion Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:DDA and AcquireX methods for TAG identification and Parallel reaction monitoring for CE identification in nonpolar extracts in positive ion mode
Ion Mode:UNSPECIFIED
  
MS ID:MS002623
Analysis ID:AN002830
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:DDA method on polar extracts in positive and negative ion mode
Ion Mode:UNSPECIFIED
  
MS ID:MS002624
Analysis ID:AN002831
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:DDA and DDA with with inclusion list for acylcarnitines on polar extracts in positive ion mode
Ion Mode:POSITIVE
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